Essentiality of CREBBP in EP300 truncated B-cell lymphoma revealed by genome-wide CRISPR-Cas9 screen

• Published in: bioRxiv
• Main Authors: Nie, Man, Du, Likun, Zhang, Bo, Ren, Weicheng, Joung, Julia, Ye, Xiaofei, Jonathan Arias Fuenzalida, Shi, Xi, Liu, Dongbing, Wu, Kui, Zhang, Feng, Pan-Hammarström, Qiang
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 28.08.2019
• Subjects: B-cell lymphoma; Copy number; CRISPR; Gene expression; Genomes; Genotypes; Lymphocytes B; Lymphoma; MDM2 protein; Oxidative phosphorylation; Phosphorylation; Tumor cell lines

Abstract Histone acetyltransferases (HATs), including CREBBP and EP300, are frequently mutated in B-cell malignancies and usually play a tumor-suppressive role. In this study, we performed whole genome and transcriptome sequencing and a genome-wide CRISPR-Cas9 knockout screen to study a germinal cen...