Chemiluminescent and Colorimetric Aptamer-Based Assays of Human [alpha]-Thrombin

A microplate-based sandwich assay for the determination of α-human thrombin (HTb) was developed. Fluorescein-modified 29-mer thrombin binding aptamer (FAM-TBA29) and biotinylated 15-mer thrombin binding aptamer (Bio-TBA15) reacting with different exosites of HTb were used as the biorecognition compo...

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Bibliographic Details
Published inAnalytical letters Vol. 53; no. 1; p. 140
Main Authors Kurseev, Sergei A, Solovjev, Anton M, Neumann, Marina M, Medvedko, Alexey V, Sakharov, Ivan Yu
Format Journal Article
LanguageEnglish
Published Abingdon Taylor & Francis Ltd 01.01.2020
Subjects
Antibodies
Assaying
Binding
Chemiluminescence
Colorimetry
Fluorescein
Laboratory equipment
Organic chemistry
Signalling systems
Thrombin
Online AccessGet full text
ISSN0003-2719
1532-236X
DOI10.1080/00032719.2019.1640718

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