The antimalarial natural product salinipostin A identifies essential α/β serine hydrolases involved in lipid metabolism in P. falciparum parasites

• Published in: bioRxiv
• Main Authors: Yoo, Euna, Schulze, Christopher J, Stokes, Barbara H, Ouma Onguka, Yeo, Tomas, Mok, Sachel, Gnadig, Nina F, Zhou, Yani, Kurita, Kenji, Foe, Ian T, Terrell, Stephanie M, Boucher, Michael J, Cieplak, Piotr, Linington, Roger G, Long, Jonathan Z, Uhlemann, Anne-Catrin, Weerapana, Eranthie, Fidock, David A, Bogyo, Matthew
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 01.11.2019
• Subjects: Antimalarial agents; Drug resistance; Esters; Homology; Hydrolase; Lipase; Lipid metabolism; Lipids; Metabolism; Natural products; Parasite resistance; Parasites; Plasmodium falciparum; Schizonts; Serine; Serine hydrolase

Salinipostin A (Sal A) is a potent antimalarial marine natural product with an undefined mechanism of action. Using a Sal A-derived activity-based probe, we identify its targets in the Plasmodium falciparum parasite. All of the identified proteins contain α/β serine hydrolase domains, and several ar...