Altered mitochondrial apparent affinity for ADP and impaired function of mitochondrial creatine kinase in gluteus medius of patients with hip osteoarthritis
The cellular energy metabolism in human musculus gluteus medius (MGM) under normal conditions and hip osteoarthritis (OA) was explored. The functions of oxidative phosphorylation and energy transport systems were analyzed in permeabilized (skinned) muscle fibers by oxygraphy, in relation to myosin h...
Saved in:
Published in | American journal of physiology. Regulatory, integrative and comparative physiology Vol. 59; no. 5; p. R1271 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Bethesda
American Physiological Society
01.05.2006
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | The cellular energy metabolism in human musculus gluteus medius (MGM) under normal conditions and hip osteoarthritis (OA) was explored. The functions of oxidative phosphorylation and energy transport systems were analyzed in permeabilized (skinned) muscle fibers by oxygraphy, in relation to myosin heavy chain (MHC) isoform distribution profile analyzed by SDS-PAGE, and to creatine kinase (CK) and adenylate kinase (AK) activities measured spectrophotometrically in the intact muscle. The results revealed high apparent Km for ADP in regulation of respiration that decreased after addition of creatine in MGM of traumatic patients (controls). OA was associated with increased sensitivity of mitochondrial respiration to ADP, decreased total activities of AK and CK with major reduction in mi-CK fraction, and attenuated effect of creatine on apparent Km for ADP compared with control group. It also included a complete loss of type II fibers in a subgroup of patients with the severest disease grade. It is concluded that energy metabolism in MGM cells is organized into functional complexes of mitochondria and ATPases. It is suggested that because of degenerative remodeling occurring during development of OA, these complexes become structurally and functionally impaired, which results in increased access of exogenous ADP to mitochondria and dysfunction of CK-phosphotransfer system.[PUBLICATION ABSTRACT] |
---|---|
AbstractList | The cellular energy metabolism in human musculus gluteus medius (MGM) under normal conditions and hip osteoarthritis (OA) was explored. The functions of oxidative phosphorylation and energy transport systems were analyzed in permeabilized (skinned) muscle fibers by oxygraphy, in relation to myosin heavy chain (MHC) isoform distribution profile analyzed by SDS-PAGE, and to creatine kinase (CK) and adenylate kinase (AK) activities measured spectrophotometrically in the intact muscle. The results revealed high apparent Km for ADP in regulation of respiration that decreased after addition of creatine in MGM of traumatic patients (controls). OA was associated with increased sensitivity of mitochondrial respiration to ADP, decreased total activities of AK and CK with major reduction in mi-CK fraction, and attenuated effect of creatine on apparent Km for ADP compared with control group. It also included a complete loss of type II fibers in a subgroup of patients with the severest disease grade. It is concluded that energy metabolism in MGM cells is organized into functional complexes of mitochondria and ATPases. It is suggested that because of degenerative remodeling occurring during development of OA, these complexes become structurally and functionally impaired, which results in increased access of exogenous ADP to mitochondria and dysfunction of CK-phosphotransfer system.[PUBLICATION ABSTRACT] |
Author | Alev, Karin Seppet, Evelin Eimre, Margus Raivo Puhke |
Author_xml | – sequence: 1 givenname: Margus surname: Eimre fullname: Eimre, Margus – sequence: 2 fullname: Raivo Puhke – sequence: 3 givenname: Karin surname: Alev fullname: Alev, Karin – sequence: 4 givenname: Evelin surname: Seppet fullname: Seppet, Evelin |
BookMark | eNqNjc1OwzAQhC3USqSFd1hxj-TYaSHHih9x5MC9spI12TZZG3sjxLvwsBiJEydOc5jvm9moFQfGC1U1O2Pqpu30SlXa7m29b5ruUm1yPmmtW9vaSn0dJsGEA8wkoR8DD4ncBC5Gl5AFnPfEJJ_gQ4LDwws4HoDm6OhH8gv3QoEh-D8DfUInxAhnYpcRiOFtWgSXDDMOVKIosSDlJMMHyQgjRQhZMLgkYyKhfKXW3k0Zr39zq26eHl_vn-uYwvuCWY6nsCQu1dGY7rYzd3pn_wV9A69IXxg |
CODEN | AJPRDO |
ContentType | Journal Article |
Copyright | Copyright American Physiological Society May 2006 |
Copyright_xml | – notice: Copyright American Physiological Society May 2006 |
DBID | 7QP 7QR 7TS 7U7 8FD C1K FR3 P64 |
DatabaseName | Calcium & Calcified Tissue Abstracts Chemoreception Abstracts Physical Education Index Toxicology Abstracts Technology Research Database Environmental Sciences and Pollution Management Engineering Research Database Biotechnology and BioEngineering Abstracts |
DatabaseTitle | Technology Research Database Toxicology Abstracts Chemoreception Abstracts Engineering Research Database Calcium & Calcified Tissue Abstracts Physical Education Index Biotechnology and BioEngineering Abstracts Environmental Sciences and Pollution Management |
DatabaseTitleList | Technology Research Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Anatomy & Physiology |
EISSN | 1522-1490 |
ExternalDocumentID | 1037124031 |
GroupedDBID | 23M 2WC 39C 4.4 5GY 5VS 6J9 7QP 7QR 7TS 7U7 8FD AAFWJ ACIWK ACPRK ADBBV AFFNX AFRAH ALMA_UNASSIGNED_HOLDINGS BAWUL BKOMP BTFSW C1A C1K DIK EBS EJD EMOBN F5P FR3 H13 ITBOX KQ8 OK1 P2P P64 PQQKQ RAP RHF RHI RPL RPRKH TR2 UKR W8F WH7 WOQ XSW YSK ~02 |
ID | FETCH-proquest_journals_2297928053 |
ISSN | 0363-6119 |
IngestDate | Fri Sep 13 07:52:12 EDT 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 5 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-proquest_journals_2297928053 |
PQID | 229792805 |
PQPubID | 48263 |
ParticipantIDs | proquest_journals_229792805 |
PublicationCentury | 2000 |
PublicationDate | 20060501 |
PublicationDateYYYYMMDD | 2006-05-01 |
PublicationDate_xml | – month: 05 year: 2006 text: 20060501 day: 01 |
PublicationDecade | 2000 |
PublicationPlace | Bethesda |
PublicationPlace_xml | – name: Bethesda |
PublicationTitle | American journal of physiology. Regulatory, integrative and comparative physiology |
PublicationYear | 2006 |
Publisher | American Physiological Society |
Publisher_xml | – name: American Physiological Society |
SSID | ssj0004343 |
Score | 3.6668122 |
Snippet | The cellular energy metabolism in human musculus gluteus medius (MGM) under normal conditions and hip osteoarthritis (OA) was explored. The functions of... |
SourceID | proquest |
SourceType | Aggregation Database |
StartPage | R1271 |
SubjectTerms | Arthritis Metabolism Muscular system |
Title | Altered mitochondrial apparent affinity for ADP and impaired function of mitochondrial creatine kinase in gluteus medius of patients with hip osteoarthritis |
URI | https://www.proquest.com/docview/229792805/abstract/ |
Volume | 59 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1bS-tAEF56fPLlcLxxvDKI-CIRk7S5PBatiNeeY4W-lTTN1kWbFG0E_S3-Qf-FM3tJExVRX0JI2CWb-XZnZuebWca2bJ4M7MjxrLDux1bdc7nVjwPbCiMe-Dx0I0eW2Dg7946u6sfdRrdWeymxlvJJfzd--jCv5CdSxWcoV8qS_YZki07xAd6jfPGKEsbrl2TcpFA3WowjnJa4jKUDeQRHNCZWOVHHORep0JTM5kFbBQpw_gtqRBrNmIvVDpQhidbnjUhRydGWyBCHkeT3MhSfS_aHLsiqs-OI8kXpIhl-5bWsk1S2eouwUKlOhdxSkXv6uyjjIZ0ilqmQvqlgQZwmnXVXFCiftipcATHSu-vR3TCfMvcj8ZDttPPrmymmb_H_qvw3UUyJy2Q8VvGY1gOl5r_dBWlUGCVmHG3zGRLimvlaWlMpbO3ZepVO9JqP_jg6intlpaDLlIty2F2u8P9tR50ZU63dfX7RO7w6Pe11Wt1O9a12tVzfptKH6Kj_cm3inp78K5W1dxW903zdO8NAWjudP-y3dlOgqTA3x2pJOs8WmikKafQI21D8gMcF9qxhCBUUgYEhGBgCwhAQhoBCBQNDMDCEjL_pwMAQFAxBpKBhCAqG1MTAEAiGgDCEKgwX2eZhq7N_ZJmR9jQG73uOE_qhE6B-WGIzaZYmfxn4aHgn9YYfxhFH5R4E_cCNXdfjqK0b8cBbZqufdLTy6dtVNjvF1BqbmdzlyTrampP-hpTTK7w4mXc |
link.rule.ids | 315,786,790 |
linkProvider | Colorado Alliance of Research Libraries |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Altered+mitochondrial+apparent+affinity+for+ADP+and+impaired+function+of+mitochondrial+creatine+kinase+in+gluteus+medius+of+patients+with+hip+osteoarthritis&rft.jtitle=American+journal+of+physiology.+Regulatory%2C+integrative+and+comparative+physiology&rft.au=Eimre%2C+Margus&rft.au=Raivo+Puhke&rft.au=Alev%2C+Karin&rft.au=Seppet%2C+Evelin&rft.date=2006-05-01&rft.pub=American+Physiological+Society&rft.issn=0363-6119&rft.eissn=1522-1490&rft.volume=59&rft.issue=5&rft.spage=R1271&rft.externalDBID=NO_FULL_TEXT&rft.externalDocID=1037124031 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0363-6119&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0363-6119&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0363-6119&client=summon |