Effects of propofol on the changes in myocardial Toll-like receptor 4 and TNF-α and NF-κb protein expressions and ultrastructure in ischemia-reperfusion injury in rats / 异丙酚对大鼠心肌缺血再灌注损伤中TLR-4、TNF-α和NF-κb表达及其超微结构的影响

• Published in: Xi'an jiao tong da xue xue bao. Journal of Xi'an Jiaotong University (medical sciences). Yi xue ban no. 3; p. 357
• Main Authors: Wen-hui, ZHAO, CHEN, Jun, Gui-xia, JING, LIU, Jian, Xiao-dong, DANG
• Format: Journal Article
• Language: Chinese
• Published: Xi'an Xi'an Jiaotong University 01.01.2014
• Subjects: Ischemia; Proteins; Rodents
• ISSN: 1671-8259
• DOI: 10.7652/jdyxb201403016

Objective: To investigate the effects of propofol on the changes in myocardial Toll-like receptor 4 (TLR-4) and TNF-α and NF-κb protein expressions in ischemia-reperfusion injury (I/R). Methods: Thirty healthy male SD rats weighing 250-320g were randomly divided into 3 groups (n=10 for each): Group A, sham operation; Group B, I/R; and Group C, propofol + I/R. In Groups B and C myocardial I/R was induced by occlusion of the left anterior descending artery (LAD) for 30min, followed by 120min reperfusion. In Group C propofol was given intravenously 10min before myocardial ischemia, followed by continuous infusion of propofol at 5mg/(kg·h) until the end of 120min reperfusion. In Groups A and B normal saline instead of propofol was given. The myocardial tissues were taken at the end of 120min; ultrastructural changes of myocardial cells were observed under X-ray electron microscope and the expressions of TLR-4 mRNA as well as TNF-α and NF-κb protein were determined. Results: Ultrastructural observation under elect