Selective extraction and characterization of a histidine-phosphorylated peptide using immobilized copper(II) ion affinity chromatography and matrix-assisted laser desorption/ionization

• Published in: Analytical chemistry (Washington) Vol. 75; no. 7; p. 1741
• Main Authors: Napper, Scott, Kindrachuk, Jason, Olson, Douglas J H, Ambrose, Stephen J
• Format: Journal Article
• Language: English
• Published: Washington American Chemical Society 01.04.2003
• Subjects: Chromatography; Copper; Ions; Lasers; Peptides

Phosphorylation is the predominant posttranslational modification involved in regulating enzymatic activity and mediating signal transduction in prokaryotic and eukaryotic cells. Selective enrichment of phosphorylated peptides prior to mass spectrometric analysis facilitates identification of phosph...