Characterizing the extracellular vesicle (EV) production of colorectal cancer cell lines

• Published in: Journal of extracellular vesicles Vol. 7; p. 128
• Main Authors: Szvicsek, Zsuzsanna, Oszvald, Adam, Kovacs, Istvan, Sandor, Gyongyver Orsolya, Zeold, Aniko, Kelemen, Andrea, Buzas, Edit, Wiener, Zoltan
• Format: Journal Article
• Language: English
• Published: Abingdon John Wiley & Sons, Inc 01.01.2018
• Subjects: CD63 antigen; CD81 antigen; Colorectal cancer; Colorectal carcinoma; Gene expression; Interleukin 11; Interleukin 22; Stroma; Tumor cell lines; Tumor necrosis factor; Tumorigenesis; Tumors; Hungary; Italy

Background: Colorectal cancer (CRC) is one of the most frequent causes of cancer-related death in the Western countries. CRC is a heterogeneous disease with different molecular background and clinical manifestations. Interestingly, colorectal cancer cell lines (CCCLs) can be classified into categories similarly to CRC patients. The potential use of EVs in the early diagnosis of tumours is based on the assumptions that (1) EV production increases during tumorigenesis and (2) tumour-derived EVs carry a specific molecular pattern. Here we studied the EV production of CCCLs from different CRC groups and the effect of external factors on EV production. Methods: We analysed CCCL-derived EVs by qNano and measured their EV production by bead-based methods and FACSCalibur. We also used publicly available gene expression data and we measured gene expression by RT-qPCR. Results: We observed a large heterogeneity in the EV production among CCCLs, although all of them secreted both CD81+ and CD63 + EVs. We could not detect a correlation between the EV production and the subtype or mutations of CCCLs. Furthermore, selected external factors, such as HGF, IL-11, IL-22 or TNF alpha had no major influence on the EV production. This suggests that these stroma-derived factors are not central in the elevated EV release from CRC tumour cells. Summary/Conclusion: All studied CCCLs produced EVs, however, the analysed stromal factors did not have a major influence on the EV secretion of CRC cells.