Characterizing the extracellular vesicle (EV) production of colorectal cancer cell lines
Background: Colorectal cancer (CRC) is one of the most frequent causes of cancer-related death in the Western countries. CRC is a heterogeneous disease with different molecular background and clinical manifestations. Interestingly, colorectal cancer cell lines (CCCLs) can be classified into categori...
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Published in | Journal of extracellular vesicles Vol. 7; p. 128 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Abingdon
John Wiley & Sons, Inc
01.01.2018
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Subjects | |
Online Access | Get full text |
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Summary: | Background: Colorectal cancer (CRC) is one of the most frequent causes of cancer-related death in the Western countries. CRC is a heterogeneous disease with different molecular background and clinical manifestations. Interestingly, colorectal cancer cell lines (CCCLs) can be classified into categories similarly to CRC patients. The potential use of EVs in the early diagnosis of tumours is based on the assumptions that (1) EV production increases during tumorigenesis and (2) tumour-derived EVs carry a specific molecular pattern. Here we studied the EV production of CCCLs from different CRC groups and the effect of external factors on EV production. Methods: We analysed CCCL-derived EVs by qNano and measured their EV production by bead-based methods and FACSCalibur. We also used publicly available gene expression data and we measured gene expression by RT-qPCR. Results: We observed a large heterogeneity in the EV production among CCCLs, although all of them secreted both CD81+ and CD63 + EVs. We could not detect a correlation between the EV production and the subtype or mutations of CCCLs. Furthermore, selected external factors, such as HGF, IL-11, IL-22 or TNF alpha had no major influence on the EV production. This suggests that these stroma-derived factors are not central in the elevated EV release from CRC tumour cells. Summary/Conclusion: All studied CCCLs produced EVs, however, the analysed stromal factors did not have a major influence on the EV secretion of CRC cells. |
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ISSN: | 2001-3078 |