Multifunctional GQDs-Concanavalin A@Fe^sub 3^O^sub 4^ nanocomposites for cancer cells detection and targeted drug delivery

Multifunctional nanocomposites containing intrinsic property for serving as the sensing elements as well as targeted nanoconjugates are highly preferred in various therapeutic applications. In this work, nanocomposites of graphene quantum dots (GQDs) and Fe3O4 with conjugation of lectin protein, con...

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Published inAnalytica chimica acta Vol. 1027; p. 109
Main Authors Chowdhury, Ankan Dutta, Ganganboina, Akhilesh Babu, Tsai, Yuan-chung, Chiu, Hsin-cheng, Doong, Ruey-an
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier BV 16.10.2018
Subjects
Biosensors
Cancer
Cellular biology
Concanavalin A
Conjugation
Cytotoxicity
Diagnostic software
Diagnostic systems
Doxorubicin
Drug delivery
Drug delivery systems
Endothelial cells
Image enhancement
Iron oxides
Magnetic fields
Magnetic permeability
Nanocomposites
Proteins
Quantum dots
Targeted cancer therapy
Therapeutic applications
Toxicity
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Summary:Multifunctional nanocomposites containing intrinsic property for serving as the sensing elements as well as targeted nanoconjugates are highly preferred in various therapeutic applications. In this work, nanocomposites of graphene quantum dots (GQDs) and Fe3O4 with conjugation of lectin protein, concanavalin A, to form GQD-ConA@Fe3O4 nanocomposites are developed for both detection of cancer cell and release of drugs to HeLa cells. The GQD-ConA@Fe3O4 nanocomposites deposited on Pt electrode can detect cancerous HeLa cells over normal endothelial cells with a dynamic linear range of 5 × 102 to 1 × 105 cells mL−1 with a detection limit of 273 cell mL−1. The GQD-ConA@Fe3O4 also can serve as nanocarriers for loading and delivering doxorubicin (Dox). The in vitro cell images show that the Dox concentration in HeLa cells is enhanced more than double in the presence of external magnetic field due to the incorporation of Fe3O4 in the nanocarrier. The cytotoxicity assay indicates that the susceptibility of cancerous HeLa cells to Dox is 13% higher than that of normal cells, confirming the selective role of ConA in nanocarriers. Results clearly indicate the GQD-ConA@Fe3O4 nanocomposites as a promising material for cancer cell detection and targeted Dox release toward HeLa cells which can serve as the multifunctional platform for novel cancer cell diagnostic and therapeutic applications.
ISSN:0003-2670
1873-4324