Analysis of Gene Expressing and Metabolites for Synthesising Anthocyanins Influencing the Red Color of Leaves of Camellia Cultivar / 红叶山茶品种花青素苷相关基因表达水平及代谢产物分析

• Published in: Forest research (Beijing) Vol. 30; no. 6; p. 1034
• Main Authors: 应震, 李纪元, 殷恒福, 范正琪, 倪穗, 吴斌, 吕焘
• Format: Journal Article
• Language: Chinese
• Published: Beijing Chinese Academy of Forestry 01.01.2017
• Subjects: Anthocyanins; Catechin; Color; Cultivars; Flavonoids; Gene expression; Genes; Glucosides; Leaves; Metabolic pathways; Metabolism; Metabolites; Polyphenols; Real time
• ISSN: 1001-1498
• DOI: 10.13275/j.cnki.lykxyj.2017.06.022

[Objective] By analyzing the quantitative change of structural genes and metabolite of anthocyanin in 4 growth stages, the relationship between leaf-color mutation and gene expression was systematically illustrated. [Method] The leaves of Camellia japonica ‘Jinhua Meinü’ were selected taking the leaves of C. japonica ‘Nuccio's Bella Rossa’, C. azalea, and C. japonica as control. NCBI Primer Designing Tool was used to design the primers and the gene expressing of DFR, ANS, UFGT, ANR and LAR were analyzed by real-time PCR and measured the contents of catechins, epicatechins and cyanidin 3-O-glucoside by HPLC. [Result] (1) In the four growth stages, no significant difference was found in the expressing of DFR, nevertheless, the expressing of ANS, UFGT,ANR and LAR between red and green cultivar were obviously different, which indicating the express levels of those genes of flavonoids metabolic pathways had been altered in the leaves of C. japonica ‘Jinhua Meinü’. (2) The content of polyphenols in the leaves of C.