A Simple and High Quality Method for Isolation and Extraction of Total RNA of Pholiota adipose

• Published in: Asian agricultural research Vol. 10; no. 1; pp. 40 - 44
• Main Authors: Wei, Zhimin, Li, Shunguo, Liu, Meng, Xia, Xueyan, Zhao, Yu, Zhou, Hanzhang, Guo, Chengang
• Format: Journal Article
• Language: English
• Published: Cranston Wu Chu (USA-China) Science and Culture Media Corporation 01.01.2018
• Subjects: Cancer; Electrophoresis; Gene expression; Genomes; Genomics; Methods; Nitrogen; Potassium; Quality; R&D; Research & development; Ribonucleic acid; RNA

According to the requirements of RNA-seq technology, high-quality RNA products must meet the following requirements; (i) the sample should have good integrity of RNA, and the RNA integrity number (RIN) must reach 6.8 or altove; (ii) the total content should be greater than 10 p.g (to be calculated u...