The Accumulation of ±-Zein in Transgenic Tobacco Endosperm is Stabilized by Co-expression of ²-Zein
The cysteine-poor ±-zein is the major prolamin storage protein fraction in maize endosperm and is localized in the interior of protein bodies with ´-zein, whereas the hydrophobic cysteine-rich ²- and ³-zein are found on the exterior of the PB. In transgenic tobacco endosperm expressing zein genes, ±...
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| Published in | Plant and cell physiology Vol. 45; no. 7; p. 864 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
Oxford
Oxford Publishing Limited (England)
15.07.2004
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| Online Access | Get full text |
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| Abstract | The cysteine-poor ±-zein is the major prolamin storage protein fraction in maize endosperm and is localized in the interior of protein bodies with ´-zein, whereas the hydrophobic cysteine-rich ²- and ³-zein are found on the exterior of the PB. In transgenic tobacco endosperm expressing zein genes, ±-zein was unstable unless co-expressed with ³-zein. Here we showed that ±-zein was also stabilized by ²-zein. Small accretions of ±- and ²-zeins, similar in appearance to maize protein bodies, were localized to the endoplasmic reticulum within tobacco endosperm cells. The zein proteins were also localized to protein storage vacuoles in a more dispersed pattern, suggesting that they were transported there after they were post-translationally sequestered into the ER. |
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| AbstractList | The cysteine-poor ±-zein is the major prolamin storage protein fraction in maize endosperm and is localized in the interior of protein bodies with ´-zein, whereas the hydrophobic cysteine-rich ²- and ³-zein are found on the exterior of the PB. In transgenic tobacco endosperm expressing zein genes, ±-zein was unstable unless co-expressed with ³-zein. Here we showed that ±-zein was also stabilized by ²-zein. Small accretions of ±- and ²-zeins, similar in appearance to maize protein bodies, were localized to the endoplasmic reticulum within tobacco endosperm cells. The zein proteins were also localized to protein storage vacuoles in a more dispersed pattern, suggesting that they were transported there after they were post-translationally sequestered into the ER. |
| Author | Yoho, Paula Randall Escobar, Sonja Coleman, Craig E Ogawa, Masahiro |
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| Copyright | Copyright Oxford University Press(England) Jul 15, 2004 |
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| Title | The Accumulation of ±-Zein in Transgenic Tobacco Endosperm is Stabilized by Co-expression of ²-Zein |
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