Kadar NF- Kβ Pankreas Tikus Model Type 2 Diabetes Mellitus dengan Pemberian Tepung Susu Sapi

• Published in: IJHN : Indonesian journal of human nutrition Vol. 2; no. 2; p. 91
• Main Authors: Fajar Ari Nugroho, Rizka Mayang Saputri Ginting, Nur, Diana
• Format: Journal Article
• Language: Indonesian
• Published: Malang Universitas Brawijaya 01.01.2015
• Subjects: Animal models; Apoptosis; Cell death; Cow's milk; Diabetes mellitus (non-insulin dependent); Insulin; NF-κB protein; Pancreas; Powder; Vitamin D
• ISSN: 2442-6636; 2355-3987
• DOI: 10.21776/ub.ijhn.2015.002.02.4

Type 2 Diabetes mellitus starts with the damage of pancreas cells and the loss of insulin sensitivity. Furthermore, it will lead to continuously progressive damage of pancreatic cells and worsening loss of insulin sensitivity. NF-Kb is discovered as protein transcription marker that is assumed to have a vital role in pancreatic cell apoptosis. NF-Kb has two different functions in apoptosis process. However, despite its anti-apoptosis function that it could protect the pancreas damage, NF-Kb tends to lead pancreas’s cells death. On the other hand, vitamin D from cow’s milk powder is known as a substance that can inhibit NF-Kb activity by improving insulin sensitivity, and it can rise the protection of pancreatic cells from death. This research aims to know the effect of cow’s milk powder vitamin D to NF-Kb rate on type 2 diabetes rat models. For 90 days, the rats were divided into 5 groups of experiment, two groups of control, (negative = non diabetic non treatment rats and positive = diabetic non treatment rats) and three groups of treatment that they were administrated cow’s milk powder with different doses (0,9 g/day, 1,8 g/day and 2,7 g/day). At the end, all groups were measured to know the rate of NF-Kb by Elisa method. The final result showed that there was no difference of NF-Kb rates between positive control and treatment groups (p value = 1,000; 0,086; 0,248). The reduction NF-Kb effect expected from cow’s milk powder administration was not proven. The conclusion was that cow’s milk powder could not be used to protect pancreatic damage by the mechanism of lowering NF-Kb rates.