Transcriptional regulation by the [gamma]5 subunit of a heterotrimeric G protein during adipogenesis

• Published in: The EMBO journal Vol. 18; no. 14; p. 4004
• Main Authors: Park, Jin-Gyoon, Muise, Aleixo, Gong-Ping, He, Sung-Woo, Kim, Hyo-Sung Ro
• Format: Journal Article
• Language: English
• Published: Heidelberg Blackwell Publishing Ltd 15.07.1999
• ISSN: 0261-4189

The adipocyte enhancer-binding protein (AEBP1) is a novel transcriptional repressor with carboxypeptidase activity. A two-hybrid screen was conducted to identify components of AEBP1 that might be important in regulating its activity. The [gamma]5 subunit of a heterotrimeric G protein was shown to bind specifically to AEBP1 and to attenuate its transcriptional repression activity. Adipogenic stimulation selectively decreased the G[gamma]5 level and enhanced the transcriptional repression activity of AEBP1 during mitotic clonal expansion at the onset of adipogenesis. Thus, the actions of G[gamma]5 and AEBP1 are directly linked, which could provide the basis for the regulation of transcription at the onset of differentiation. This report shows that a signal-transducing molecule is involved, by direct protein-protein interaction, in the regulation of transcription during adipogenesis.