Activity of a novel PDGF [beta]-receptor enhancer during the cell cycle and upon differentiation of neuroblastoma
PDGF acts as an autocrine and paracrine factor in certain tumors through upregulation of the PDGF [gamma]-receptor expression. In order to elucidate the control mechanism for the receptor expression, we have isolated an enhancer from two P1 clones that together contain a 102 kb NotI region covering...
Saved in:
Published in | Experimental cell research Vol. 312; no. 11; p. 2028 |
---|---|
Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
New York
Elsevier BV
01.07.2006
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | PDGF acts as an autocrine and paracrine factor in certain tumors through upregulation of the PDGF [gamma]-receptor expression. In order to elucidate the control mechanism for the receptor expression, we have isolated an enhancer from two P1 clones that together contain a 102 kb NotI region covering the entire human PDGFRB gene. They were partially digested with TspI and cloned into the PDGFRB enhancer trap vector to make a library for identification of enhancers. The digested DNA containing enhancer was identified by expression of GFP when transfected in PDGF [gamma]-receptor expressing cells. One of the enhancer clones was further examined by making several deletion mutants in a luciferase vector. This enhancer was most active in neuroblastoma cells, IMR32 and BE2, but less active in hemangioma and in smooth muscle cell lines. Chip assay revealed that SP1, AP2, and GATA2 bound the enhancer in BE2 cells. Their interaction occurred dependently of the cell cycle and synchronously with their binding to the promoter. Transfection of GATA2 alone or with Ets, which binds adjacent to GATA, resulted in differentiation of BE2 cells in parallel with increased PDGF [gamma]-receptor expression. Furthermore, over-expression of the PDGF [gamma]-receptor in BE2 cells induced neurite extension. [PUBLICATION ABSTRACT] |
---|---|
AbstractList | PDGF acts as an autocrine and paracrine factor in certain tumors through upregulation of the PDGF [gamma]-receptor expression. In order to elucidate the control mechanism for the receptor expression, we have isolated an enhancer from two P1 clones that together contain a 102 kb NotI region covering the entire human PDGFRB gene. They were partially digested with TspI and cloned into the PDGFRB enhancer trap vector to make a library for identification of enhancers. The digested DNA containing enhancer was identified by expression of GFP when transfected in PDGF [gamma]-receptor expressing cells. One of the enhancer clones was further examined by making several deletion mutants in a luciferase vector. This enhancer was most active in neuroblastoma cells, IMR32 and BE2, but less active in hemangioma and in smooth muscle cell lines. Chip assay revealed that SP1, AP2, and GATA2 bound the enhancer in BE2 cells. Their interaction occurred dependently of the cell cycle and synchronously with their binding to the promoter. Transfection of GATA2 alone or with Ets, which binds adjacent to GATA, resulted in differentiation of BE2 cells in parallel with increased PDGF [gamma]-receptor expression. Furthermore, over-expression of the PDGF [gamma]-receptor in BE2 cells induced neurite extension. [PUBLICATION ABSTRACT] |
Author | Watt, Fujiko Kaneko, Masaharu Yang, Weiwen Matsumoto, Yoshiki Funa, Keiko |
Author_xml | – sequence: 1 givenname: Masaharu surname: Kaneko fullname: Kaneko, Masaharu – sequence: 2 givenname: Weiwen surname: Yang fullname: Yang, Weiwen – sequence: 3 givenname: Yoshiki surname: Matsumoto fullname: Matsumoto, Yoshiki – sequence: 4 givenname: Fujiko surname: Watt fullname: Watt, Fujiko – sequence: 5 givenname: Keiko surname: Funa fullname: Funa, Keiko |
BookMark | eNqNjLFOwzAURS1UJFLgC1ie2GOe3ZCmIwIKIwMbQpXrvFBH5jm1nYr8PRn4AKaro3N0l2LBgUmIG4VSoarvejnRj41SI9YSVxLx_kwUCjdY6krrhSgQVVVWjV5fiGVKPSI2jaoLcXyw2Z1cniB0YIDDiTy8Pb1s4WNP2XyWkSwNOUQgPhi2FKEdo-MvyAcCS96DnawnMNzCOASG1nUdReLsTHYzz79MYwx7b1IO3-ZKnHfGJ7r-20txu31-f3wthxiOI6W868MYeVY7tanqdaMUrv4V_QILiFRL |
ContentType | Journal Article |
Copyright | Copyright © 2006 Elsevier B.V. All rights reserved. |
Copyright_xml | – notice: Copyright © 2006 Elsevier B.V. All rights reserved. |
DBID | 7TK 7TM 8FD FR3 P64 RC3 |
DOI | 10.1016/j.yexcr.2006.03.005 |
DatabaseName | Neurosciences Abstracts Nucleic Acids Abstracts Technology Research Database Engineering Research Database Biotechnology and BioEngineering Abstracts Genetics Abstracts |
DatabaseTitle | Genetics Abstracts Technology Research Database Nucleic Acids Abstracts Engineering Research Database Neurosciences Abstracts Biotechnology and BioEngineering Abstracts |
DatabaseTitleList | Genetics Abstracts |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Biology |
EISSN | 1090-2422 |
ExternalDocumentID | 1068238721 |
Genre | Feature |
GroupedDBID | --- --K --M -DZ -~X .55 .GJ .~1 0R~ 1B1 1RT 1~. 1~5 3O- 4.4 457 4G. 53G 5GY 5RE 5VS 7-5 71M 7TK 7TM 8FD 8P~ 9JM 9M8 AABNK AACTN AAEDT AAEDW AAIKJ AAKOC AALRI AAOAW AAQFI AAXKI AAXUO ABFNM ABFRF ABGSF ABJNI ABMAC ABOCM ABPPZ ABUDA ABXDB ACDAQ ACGFO ACGFS ACNCT ACPRK ACRLP ADBBV ADEZE ADMUD ADUVX ADVLN AEBSH AEFWE AEHWI AEKER AENEX AFKWA AFTJW AFXIZ AGUBO AGYEJ AHHHB AIEXJ AIKHN AITUG AJOXV AKRWK ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ AXJTR BKOJK BLXMC C45 CS3 DM4 DU5 EBS EFBJH EJD EO8 EO9 EP2 EP3 F5P FDB FIRID FNPLU FR3 FYGXN G-Q G8K HZ~ IHE J1W K-O KOM L7B LG5 LX2 M41 MO0 N9A O-L O9- OAUVE OVD OZT P-9 P2P P64 PC. PQQKQ Q38 RC3 RIG RNS ROL RPZ SCC SDF SDG SDP SES SPCBC SSU SSZ T5K TEORI TWZ VQA WH7 X7M Y6R YZZ ZCA ZMT ZU3 ~G- ~KM |
ID | FETCH-proquest_journals_1946781103 |
ISSN | 0014-4827 |
IngestDate | Fri Sep 13 01:51:17 EDT 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 11 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-proquest_journals_1946781103 |
PQID | 194678110 |
PQPubID | 32173 |
ParticipantIDs | proquest_journals_194678110 |
PublicationCentury | 2000 |
PublicationDate | 20060701 |
PublicationDateYYYYMMDD | 2006-07-01 |
PublicationDate_xml | – month: 07 year: 2006 text: 20060701 day: 01 |
PublicationDecade | 2000 |
PublicationPlace | New York |
PublicationPlace_xml | – name: New York |
PublicationTitle | Experimental cell research |
PublicationYear | 2006 |
Publisher | Elsevier BV |
Publisher_xml | – name: Elsevier BV |
SSID | ssj0008816 |
Score | 3.6869261 |
Snippet | PDGF acts as an autocrine and paracrine factor in certain tumors through upregulation of the PDGF [gamma]-receptor expression. In order to elucidate the... |
SourceID | proquest |
SourceType | Aggregation Database |
StartPage | 2028 |
SubjectTerms | Cell cycle Gene expression Genomics T cell receptors Tumors |
Title | Activity of a novel PDGF [beta]-receptor enhancer during the cell cycle and upon differentiation of neuroblastoma |
URI | https://www.proquest.com/docview/194678110/abstract/ |
Volume | 312 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1JS8NAFB6KIngRV9wZRE8lpUk7aXp0q-J-qFoRKZM4pWkl0TZR68G_5F_0vZlstSLqJZQEXpK-L29fCNnkZa5XSrauCcfgWpnjN2cUq5rTAg_XsmzwOLA5-fTMPLwsHzVYI5f7yFQthYFdcN6-7Sv5D1fhHPAVu2T_wNmEKJyA38BfOAKH4fgrHm870e4H2eTo-c_iIX-xd1DLb7EdW4BdyPY0kGjiMcC53l4bOdyLOxPR4sSwfd4ZAFmZRAgfAQvxypTATaxJOfTSBjs78CMx3kkq-NL9AJJYNDwoCTIfc090fdUV1Odt3gsTORNFqq-F-5L2o53yAP5BXy53yt_4_bbbddOgv8pk1cKO2_W_xisq2XhF2khzlZXLelnDgaRKKylRXKwWMWE9JKtLupEFpZ4VvUXVZT6iE1R4olMYiFenF6WfcKwtS1VgnPY_O2_WLk9OmvX9Rn34auQwmRaYOBUcXDBuVKoMvf3Ce1pTZFly127yOvGYK1lQOHL_EfUvbZr6NJmKnBG6rZA1Q3LCmyUTaj3pYI48xfiifotyKvFFEV_0FtF1l2CLxtiiClsUsEURDlRiiwK2KGKLfsEW0h3C1jzZqO3Xdw-1-Imb0XfQb-pVULYWmJGlBTLm-Z5YJNSwGbNMG6sFOFg8wmLcNO6ZYCbnmOteIis_EFr-8eoKmTTiQkx9lYwFvVCsgWUY2OuSG5_j62x1 |
link.rule.ids | 315,786,790,27957,27958 |
linkProvider | Elsevier |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Activity+of+a+novel+PDGF+%5Bbeta%5D-receptor+enhancer+during+the+cell+cycle+and+upon+differentiation+of+neuroblastoma&rft.jtitle=Experimental+cell+research&rft.au=Kaneko%2C+Masaharu&rft.au=Yang%2C+Weiwen&rft.au=Matsumoto%2C+Yoshiki&rft.au=Watt%2C+Fujiko&rft.date=2006-07-01&rft.pub=Elsevier+BV&rft.issn=0014-4827&rft.eissn=1090-2422&rft.volume=312&rft.issue=11&rft.spage=2028&rft_id=info:doi/10.1016%2Fj.yexcr.2006.03.005&rft.externalDBID=NO_FULL_TEXT&rft.externalDocID=1068238721 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0014-4827&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0014-4827&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0014-4827&client=summon |