Directed evolution of the tryptophan synthase [Beta]-subunit for stand-alone function recapitulates allosteric activation

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 112; no. 47; p. 14599
• Main Authors: Buller, Andrew R, Brinkmann-Chen, Sabine, Romney, David K, Herger, Michael, Murciano-Calles, Javier, Arnold, Frances H
• Format: Journal Article
• Language: English
• Published: Washington National Academy of Sciences 24.11.2015
• Subjects: Amino acids; Chemical reactions; Enzymes; Evolution; Kinetics; Microorganisms

Enzymes in heteromeric, allosterically regulated complexes catalyze a rich array of chemical reactions. Separating the subunits of such complexes, however, often severely attenuates their catalytic activities, because they can no longer be activated by their protein partners. We used directed evolut...