Temporal Dynamics of Intrahost Molecular Evolution for a Plant RNA Virus
Populations of plant RNA viruses are highly polymorphic in infected plants, which may allow rapid within-host evolution. To understand tobacco etch potyvirus (TEV) evolution, longitudinal samples from experimentally evolved populations in the natural host tobacco and from the alternative host pepper...
Saved in:
Published in | Molecular biology and evolution Vol. 32; no. 5; p. 113 |
---|---|
Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford
Oxford Publishing Limited (England)
01.05.2015
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Populations of plant RNA viruses are highly polymorphic in infected plants, which may allow rapid within-host evolution. To understand tobacco etch potyvirus (TEV) evolution, longitudinal samples from experimentally evolved populations in the natural host tobacco and from the alternative host pepper were phenotypically characterized and genetically analyzed. Temporal and compartmental variabilities of TEV populations were quantified using high throughput Illumina sequencing and population genetic approaches. Of the two viral phenotypic traits measured, virulence increased in the novel host but decreased in the original one, and viral load decreased in both hosts, though to a lesser extent in the novel one. Dynamics of population genetic diversity were also markedly different among hosts. Population heterozygosity increased in the ancestral host, with a dominance of synonymous mutations fixed, whereas it did not change or even decreased in the new host, with an excess of nonsynonymous mutations. All together, these observations suggest that directional selection is the dominant evolutionary force in TEV populations evolving in a novel host whereas either diversifying selection or random genetic drift may play a fundamental role in the natural host. To better understand these evolutionary dynamics, we developed a computer simulation model that incorporates the effects of mutation, selection, and drift. Upon parameterization with empirical data from previous studies, model predictions matched the observed patterns, thus reinforcing our idea that the empirical patterns of mutation accumulation represent adaptive evolution. |
---|---|
AbstractList | Populations of plant RNA viruses are highly polymorphic in infected plants, which may allow rapid within-host evolution. To understand tobacco etch potyvirus (TEV) evolution, longitudinal samples from experimentally evolved populations in the natural host tobacco and from the alternative host pepper were phenotypically characterized and genetically analyzed. Temporal and compartmental variabilities of TEV populations were quantified using high throughput Illumina sequencing and population genetic approaches. Of the two viral phenotypic traits measured, virulence increased in the novel host but decreased in the original one, and viral load decreased in both hosts, though to a lesser extent in the novel one. Dynamics of population genetic diversity were also markedly different among hosts. Population heterozygosity increased in the ancestral host, with a dominance of synonymous mutations fixed, whereas it did not change or even decreased in the new host, with an excess of nonsynonymous mutations. All together, these observations suggest that directional selection is the dominant evolutionary force in TEV populations evolving in a novel host whereas either diversifying selection or random genetic drift may play a fundamental role in the natural host. To better understand these evolutionary dynamics, we developed a computer simulation model that incorporates the effects of mutation, selection, and drift. Upon parameterization with empirical data from previous studies, model predictions matched the observed patterns, thus reinforcing our idea that the empirical patterns of mutation accumulation represent adaptive evolution. |
Author | Willemsen, Anouk Cuevas, José M Elena, Santiago F Zwart, Mark P Hillung, Julia |
Author_xml | – sequence: 1 givenname: José surname: Cuevas middlename: M fullname: Cuevas, José M – sequence: 2 givenname: Anouk surname: Willemsen fullname: Willemsen, Anouk – sequence: 3 givenname: Julia surname: Hillung fullname: Hillung, Julia – sequence: 4 givenname: Mark surname: Zwart middlename: P fullname: Zwart, Mark P – sequence: 5 givenname: Santiago surname: Elena middlename: F fullname: Elena, Santiago F |
BookMark | eNqNissKgkAUQIcwSKt_uNBamMH3MsqwRREhbWWQkZRxrs0j6O9z0Qe0OgfOCYinUIkF8VkSZSHLWOERn2azxzTKVyQwZqCUxXGa-qSqxTih5hKOH8XHvjWAHZyV1fyJxsIFpWid5BrKN0pne1TQoQYON8mVhft1D49eO7Mhy45LI7Y_rsnuVNaHKpw0vpwwthnQaTWnhqU5S2iUFnH03_UFdw4-fw |
ContentType | Journal Article |
Copyright | Copyright Oxford Publishing Limited(England) May 2015 |
Copyright_xml | – notice: Copyright Oxford Publishing Limited(England) May 2015 |
DBID | 7QG 7QP 7QR 7SN 7SS 7TK 7TM 7TO 7U9 8FD C1K FR3 H94 K9. M7N P64 RC3 |
DatabaseName | Animal Behavior Abstracts Calcium & Calcified Tissue Abstracts Chemoreception Abstracts Ecology Abstracts Entomology Abstracts (Full archive) Neurosciences Abstracts Nucleic Acids Abstracts Oncogenes and Growth Factors Abstracts Virology and AIDS Abstracts Technology Research Database Environmental Sciences and Pollution Management Engineering Research Database AIDS and Cancer Research Abstracts ProQuest Health & Medical Complete (Alumni) Algology Mycology and Protozoology Abstracts (Microbiology C) Biotechnology and BioEngineering Abstracts Genetics Abstracts |
DatabaseTitle | Virology and AIDS Abstracts Oncogenes and Growth Factors Abstracts Technology Research Database Nucleic Acids Abstracts ProQuest Health & Medical Complete (Alumni) Ecology Abstracts Neurosciences Abstracts Biotechnology and BioEngineering Abstracts Environmental Sciences and Pollution Management Entomology Abstracts Genetics Abstracts Animal Behavior Abstracts Algology Mycology and Protozoology Abstracts (Microbiology C) AIDS and Cancer Research Abstracts Chemoreception Abstracts Engineering Research Database Calcium & Calcified Tissue Abstracts |
DatabaseTitleList | Virology and AIDS Abstracts |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Biology |
EISSN | 1537-1719 |
ExternalDocumentID | 3688330501 |
Genre | Feature |
GroupedDBID | --- -E4 -~X .2P .I3 .ZR 0R~ 18M 1TH 29M 2WC 4.4 48X 5VS 5WA 70D 7QG 7QP 7QR 7SN 7SS 7TK 7TM 7TO 7U9 8FD AAFWJ AAIJN AAIMJ AAJKP AAJQQ AAMDB AAMVS AAOGV AAPNW AAPQZ AAPXW AAUQX AAVAP AAVLN ABEUO ABIXL ABKDP ABLJU ABNKS ABPTD ABQLI ABQTQ ABXVV ABZBJ ACGFO ACGFS ACIPB ACIWK ACNCT ACPRK ACUFI ACUTO ACYTK ADBBV ADEYI ADEZT ADFTL ADGZP ADHKW ADHZD ADJQC ADOCK ADRIX ADRTK ADYVW ADZTZ ADZXQ AECKG AEGPL AEJOX AEKKA AEKSI AELWJ AEMDU AENEX AENZO AEPUE AETBJ AEWNT AFIYH AFOFC AFPKN AFRAH AFULF AFXEN AGINJ AGKEF AGSYK AHMBA AHXPO AIAGR AIJHB AJEUX AKHUL AKWXX ALMA_UNASSIGNED_HOLDINGS ALTZX ALUQC APIBT APWMN ARIXL AXUDD AYOIW AZVOD BAWUL BAYMD BCRHZ BEYMZ BHONS BQDIO BQUQU BSWAC BTQHN BTRTY BVRKM C1K CDBKE CS3 CZ4 DAKXR DIK DILTD DU5 D~K E3Z EBS EE~ EJD EMOBN F5P F9B FHSFR FLIZI FOTVD FR3 GAUVT GJXCC GROUPED_DOAJ GX1 H13 H5~ H94 HAR HH5 HW0 HZ~ IOX J21 K9. KOP KQ8 KSI KSN M-Z M49 M7N ML0 N9A NGC NLBLG NMDNZ NOYVH NU- O9- OAWHX ODMLO OJQWA OK1 OVD P2P P64 PAFKI PEELM PQQKQ Q1. Q5Y RC3 RD5 RHF ROL ROX ROZ RPM RUSNO RW1 RXO TEORI TJP TJX TLC TN5 TOX TR2 VQA W8F WOQ X7H XSW YAYTL YKOAZ YXANX ZCA ZKX ~02 ~91 |
ID | FETCH-proquest_journals_16815036943 |
ISSN | 0737-4038 |
IngestDate | Fri Sep 13 05:49:30 EDT 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 5 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-proquest_journals_16815036943 |
PQID | 1681503694 |
PQPubID | 36253 |
ParticipantIDs | proquest_journals_1681503694 |
PublicationCentury | 2000 |
PublicationDate | 20150501 |
PublicationDateYYYYMMDD | 2015-05-01 |
PublicationDate_xml | – month: 05 year: 2015 text: 20150501 day: 01 |
PublicationDecade | 2010 |
PublicationPlace | Oxford |
PublicationPlace_xml | – name: Oxford |
PublicationTitle | Molecular biology and evolution |
PublicationYear | 2015 |
Publisher | Oxford Publishing Limited (England) |
Publisher_xml | – name: Oxford Publishing Limited (England) |
SSID | ssj0014466 |
Score | 4.3704557 |
Snippet | Populations of plant RNA viruses are highly polymorphic in infected plants, which may allow rapid within-host evolution. To understand tobacco etch potyvirus... |
SourceID | proquest |
SourceType | Aggregation Database |
StartPage | 113 |
SubjectTerms | Evolutionary biology Genetic diversity Mutation Population genetics Ribonucleic acid RNA Viruses |
Title | Temporal Dynamics of Intrahost Molecular Evolution for a Plant RNA Virus |
URI | https://www.proquest.com/docview/1681503694/abstract/ |
Volume | 32 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LS8NAEF5qQfAiPtFaZUFvoSXp5tEci7QEqS1IKsFLSNINeDCBNqnor3c2-0iKUtRLKFm6LJkv305m9ptB6M6x9SSp0u4JUCArcd5zaer0Yp2SeBjRdGkx7fDjzPYW5kNgBa3WpnFqqSzifvL5o67kP1aFe2BXppL9g2XVpHADfoN94QoWhuvvbMzrSgFx8b7yayEMAQLJ1wW8r6L1rTbeiHXwQ5NVq6JCe5qNtOfXlfj0l22d1J-a9ZmonEDlLEq6iWQOgWfbta0gDn0TsZ1RlpdKDuTBiKAXpsxWe8LLe1Rrh4TmTMQiDKs--dfUODYCaEKlxbxl0ZREBjg4xTkEAKLz-i59KimYla0URCo4uo6B1lnwinANrmTdLqQ9m4eTxXQa-uPA3x7lG7fNOizrFpP97RGDHQT154HKPckMt1zbt126cj38I3QovhnwiAPgGLVodoL2eRfRj1PkSRhgCQOcp1jBACuLYgUDDE8QR7iCAQYY4AoGZ-h2MvbvvZ5cSChQuA4Newg-PbFdk5yjdpZn9ALhgUn02DRJZMaWuXSIa7tJCr7kcmCDhz3QL1F310yd3cNX6KA2fhe1i1VJr8E1K-Kb6kl-ARKwQ90 |
link.rule.ids | 315,786,790 |
linkProvider | Oxford University Press |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Temporal+Dynamics+of+Intrahost+Molecular+Evolution+for+a+Plant+RNA+Virus&rft.jtitle=Molecular+biology+and+evolution&rft.au=Cuevas%2C+Jos%C3%A9+M&rft.au=Willemsen%2C+Anouk&rft.au=Hillung%2C+Julia&rft.au=Zwart%2C+Mark+P&rft.date=2015-05-01&rft.pub=Oxford+Publishing+Limited+%28England%29&rft.issn=0737-4038&rft.eissn=1537-1719&rft.volume=32&rft.issue=5&rft.spage=113&rft.externalDBID=NO_FULL_TEXT&rft.externalDocID=3688330501 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0737-4038&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0737-4038&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0737-4038&client=summon |