Enhancement ofin vivoandin vitroimmune functions by a conformationally biased, response-selective agonist of human C5a: Implications for a novel adjuvant in vaccine design
A conformationally biased, agonist of human C5a65-74(EP67) was assessed for its adjuvant activitiesin vitroandin vivo. EP67 induced the release of the inflammatory (Th1) type cytokines from C5a receptor (CD88)-bearing antigen presenting cells (APC). EP67 did not induce the release of these cytokines...
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Published in | Vaccine Vol. 28; no. 2; p. 463 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Kidlington
Elsevier Limited
11.12.2009
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Subjects | |
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Abstract | A conformationally biased, agonist of human C5a65-74(EP67) was assessed for its adjuvant activitiesin vitroandin vivo. EP67 induced the release of the inflammatory (Th1) type cytokines from C5a receptor (CD88)-bearing antigen presenting cells (APC). EP67 did not induce the release of these cytokines from splenic APCs obtained from C5a receptor knockouts (CD88-/-). Serum from mice immunized with EP67-ovalbumin (OVA) contained high OVA-specific antibody (Ab) titers [IgG1, IgG2a (IGg2c), IgG2b]. Mice receiving OVA alone produced only IgG1 Abs, indicating the ability of EP67 to induce a Th1-like Ab class switch. Spleen cell cultures from wild type mice but not CD88-/-mice showed an enhanced OVA-specific proliferative responsein vitro. These results indicate the ability of EP67 to drive a Th1-mediated immune response and its potential use as a unique adjuvant. |
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AbstractList | A conformationally biased, agonist of human C5a65-74(EP67) was assessed for its adjuvant activitiesin vitroandin vivo. EP67 induced the release of the inflammatory (Th1) type cytokines from C5a receptor (CD88)-bearing antigen presenting cells (APC). EP67 did not induce the release of these cytokines from splenic APCs obtained from C5a receptor knockouts (CD88-/-). Serum from mice immunized with EP67-ovalbumin (OVA) contained high OVA-specific antibody (Ab) titers [IgG1, IgG2a (IGg2c), IgG2b]. Mice receiving OVA alone produced only IgG1 Abs, indicating the ability of EP67 to induce a Th1-like Ab class switch. Spleen cell cultures from wild type mice but not CD88-/-mice showed an enhanced OVA-specific proliferative responsein vitro. These results indicate the ability of EP67 to drive a Th1-mediated immune response and its potential use as a unique adjuvant. |
Author | Stein, Elisabeth A Sanderson, Sam D Morgan, Edward L Vitrs, Elizabeth L Thoman, Marilyn L Phillips, Joy A Morgan, Brandon N |
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DOI | 10.1016/j.vaccine.2009.10.029 |
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Snippet | A conformationally biased, agonist of human C5a65-74(EP67) was assessed for its adjuvant activitiesin vitroandin vivo. EP67 induced the release of the... |
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Title | Enhancement ofin vivoandin vitroimmune functions by a conformationally biased, response-selective agonist of human C5a: Implications for a novel adjuvant in vaccine design |
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