Development of Copper-Catalyzed Azide-Alkyne Cycloaddition for Increased in Vivo Efficacy of Interferon [Beta]-1b by Site-Specific PEGylation

• Published in: Bioconjugate chemistry Vol. 23; no. 10; p. 2087
• Main Authors: Nairn, Natalie W, Shanebeck, Kurt D, Wang, Aijun, Graddis, Thomas J, VanBrunt, Michael Pete, Thornton, Kenneth C, Grabstein, Kenneth
• Format: Journal Article
• Language: English
• Published: Washington American Chemical Society 17.10.2012
• Subjects: Amino acids; Bioavailability; Chemical reactions; Interferon; Pharmacology; Proteins; Tumors

The development of protein conjugate therapeutics requires control over the site of modification to allow for reproducible generation of a product with the desired potency, pharmacokinetic, and safety profile. Placement of a single nonnatural amino acid at the desired modification site of a recombin...