Effects of [alpha]-ketoglutarate on neutrophil intracellular amino and [alpha]-keto acid profiles and ROS production

• Published in: Amino acids Vol. 38; no. 1; p. 167
• Main Authors: Mühling, Jörg, Tussing, F, Nickolaus, K A, Matejec, R, Henrich, M, Harbach, H, Wolff, M, Weismüller, K, Engel, J, Welters, I D, Langefeld, T W, Fuchs, M, Weigand, M A, Heidt, M C
• Format: Journal Article
• Language: English
• Published: Vienna Springer Nature B.V 01.01.2010
• ISSN: 0939-4451; 1438-2199
• DOI: 10.1007/s00726-008-0224-5

The aim of this study was to determine the effects of α-ketoglutarate on neutrophil (PMN), free α-keto and amino-acid profiles as well as important reactive oxygen species (ROS) produced [superoxide anion (O^sub 2^ ^sup -^), hydrogen peroxide (H^sub 2^O^sub 2^)] and released myeloperoxidase (MPO) acitivity. Exogenous α-ketoglutarate significantly increased PMN α-ketoglutarate, pyruvate, asparagine, glutamine, asparatate, glutamate, arginine, citrulline, alanine, glycine and serine in a dose as well as duration of exposure dependent manner. Additionally, in parallel with intracellular α-ketoglutarate changes, increases in O^sub 2^ ^sup -^ formation, H^sub 2^O^sub 2^-generation and MPO acitivity have also been observed. We therefore believe that α-ketoglutarate is important for affecting PMN "susceptible free amino- and α-keto acid pools" although important mechanisms and backgrounds are not yet completely explored. Moreover, our results also show very clearly that changes in intragranulocytic α-ketoglutarate levels are relevant metabolic determinants in PMN nutrition considerably influencing and modulating the magnitude and quality of the granulocytic host defense capability as well as production of ROS.[PUBLICATION ABSTRACT]