Science And Technology Against Microbial Pathogens

• Main Author: Mendez-vilas, Antonio
• Format: eBook
• Language: English
• Published: Singapore World Scientific Publishing Company 2011
• Edition: 1
• Subjects: Congresses; Drug resistance in microorganisms; Pathogenic bacteria

Table of Contents:

• 2. Results and Discussion -- 2.1 Effect of N- vs. C-terminal 6 x His tag on mLyso lytic activity -- 2.2 Activity changes resulting from N125Q and N232Q mLyso mutations -- 2.3 N-terminal deletions of mLyso between residue 1 and 31 -- Conclusions -- References -- Purification and characterization of antimicrobial peptides from fleshfly larvae haemolymph T. Neubauerová, M. Macková, T. Macek, M. Šanda and Z. Voburka -- 1. General remarks -- 2. Materials and methods -- 2.1 Isolation of larval haemolymph -- 2.2 Purification of antimicrobial peptides -- 2.3 Ion-exchange and reversed-phase liquid chromatography (Ionex-FPLC, RP-HPLC) -- 2.4 Microorganisms and antimicrobial assay -- 2.5 Tricine gel electrophoresis and electroblotting -- 2.6 Mass spectrometry analysis -- 3. Results and discussion -- References -- Structural and functional diversity of natural antimicrobial oligopeptides Aexander A. Zamyatnin -- 1. Introduction -- 2. Structure -- 3. Functions -- 4. Potential applications -- References -- The role of Gram-negative envelope LPS on the bactericidal properties of proteins and peptides: the case of eosinophil cationic protein D. Pulido, M. Torrent, M. V. Nogués and E. Boix -- 1. General remarks -- 2. Results and discussion -- 2.1 Antimicrobial activity -- 2.2 Minimal agglutination activity (MAC) -- 2.3 Depolarization activity -- 2.4 Scanning electron microscopy (SEM) -- References -- Non-antibiotics Biocides -- Evaluation of biocidal activity of Evolyse, a disinfectant based on hydrogen peroxide and silver nitrate M. Barbara Pisano, V. Altana, M. Elisabetta Fadda, L. Mura, M. Deplano and S. Cosentino -- 1. Introduction -- 2. Materials and Methods -- 2.1 Quantitative suspension test -- 2.2 Test under practical conditions -- 3. Results -- 4. Discussion and Conclusions -- References
• 2.3 rRNA 16S amplification and DGGE gel
• 1. Introduction -- 2. Material and methods -- 2.1 Bacterial strains, reagents and growth conditions -- 2.2 Static and flow-cell biofilm assays -- 2.3 sodA and micF promoter fusion expression assays -- 3. Results and discussion -- 3.1 Curcumin inhibits E. coli and S. aureus but not P. aeruginosa biofilm formation -- 3.2 Curcumin affects established E. coli and S. aureus biofilms -- 3.3 Effect of curcumin on E. coli and S. aureus morphology -- 3.4 Curcumin induces stress related genes in E. coli -- 4. Conclusions -- References -- Evaluation of in vitro and in vivo antibacterial and antifungal activity of "Camelyn M" Benedikte Maglakelidze, Guguli Abashidze, Inga Dadeshidze, Vakxtang Mshvildadze, Andre Pichete, Vincent Perreten, Shota Tsanava, Nata Shubladze and Koba Gurielidze -- Introduction -- Materials and Methods -- Results -- References -- In vitro antifungal activity of several essential oils from aromatic plants of Aragón (NE, Spain) S. Lorán, O. Segman, C. Rota, P. Conchello and A. Herrera -- 1. Introduction -- 2. Material and methods -- 2.1 Plant material and isolation of essential oils -- 2.2 Test microorganism and growth conditions -- 2.3 Antifungal assay (disk diffusion assay) -- 3. Results and discussion -- References -- Influence of some natural compounds on freshwater microfoulants O. A. Cuzman, C. Faraloni, T. Turchetti, M. Camaiti and P. Tiano -- 1. Introduction -- 2. Materials and methods -- 2.1. Natural compounds -- 2.2. Serial dilution method -- 2.3. Diffusion method -- 3. Results and discussion -- 3.1. Serial dilution method -- 3.2. Diffusion method -- 4. Conclusion -- References -- Natural antimicrobial agents against the microbiota associated with insoles N. Cuesta Garrote, M. M. Sánchez Navarro, F. Arán Aís and C. Orgilés Barceló -- 1. Introduction -- 2. Experimental -- 2.1 Materials -- 2.2 DNA extraction
• 4.3 Effectiveness -- 4.4 Safety -- 5. Discussion -- 6. Conclusion -- References -- Natural Products: Terrestrial and Marine Organisms -- Analysis of the 2-Phenylethyl isothiocyanate present in Brassica leaves and their potential application as antimicrobial agent against bacteria strains isolated from Human and Pig gastrointestinal tracts A. Aires, C. Dias, R. N. Bennett, E. A. S. Rosa and M. J. Saavedra -- 1. Introduction -- 2. Materials and Methods -- 2.1 Glucosinolate hydrolysis product -- 2.2 Isolation, identification and growth of bacterial strains -- 2.3 Screening of antibacterial effects using a disc diffusion method -- 2.4 Statistical analysis -- 3. Results and Discussion -- References -- Antimicrobial effect of carvacrol on Escherichia coli K12 growth at different temperatures C.M. Belda-Galbis, A. Martínez and D. Rodrigo -- 1. Background -- 2. Materials and methods -- 2.1 Bacterial strain and growth conditions -- 2.2 Carvacrol -- 2.3 Antimicrobial assay -- 2.4 Modelization of E. coli K12 growth -- 2.5 Statistical data analysis -- 3. Results and discussion -- 3.1 Growth curves -- 3.2 Modeling growth and determination of kinetic parameters -- 3.3 Effect of carvacrol concentration and incubation temperature on E. coli K12 growth -- 3.4 Effect of carvacrol concentration on µ -- 4. Conclusion -- References -- Bacteriostatic effect of cocoa powder rich in polyphenols to control Cronobacter sakazakii proliferation on infant milk formula M. C. Pina-Pérez, D. Rodrigo and A. Martínez-López -- 1. Introduction -- 2. Objective -- 3. Material and Methods -- 3.1 Microorganism culture -- 3.2 Cocoa powder -- 3.3 Substrate of inoculation -- 3.4 Viable C. sakazakii cell counts -- 3.5 Modelling C. sakazakii growth -- 4. Results and Discussion -- 5. Conclusions -- References -- Curcumin: a natural antibiofilm agent Maya Moshe, Jonathan Lellouche and Ehud Banin
• Intro -- Contents -- Introduction -- Antimicrobial Peptides -- A new class of Scots pine antimicrobial proteins, which act by binding b-glucan Sanjeewani Sooriyaarachchi, Adrian Suárez Covarrubias, Wimal Ubhayasekera, Frederick O. Asiegbu and Sherry L. Mowbray -- 1. Introduction -- 2. Material and methods -- 2.1 Cloning, expression and purification of Sp-AMP3 -- 2.2 Binding studies -- 2.3 Antifungal activity -- 2.4 Scanning electron microscopic studies -- 2.5 Homology modeling -- 3. Results and discussion -- 4. Conclusions -- References -- Antimicrobial aza- -peptides: Structure-activity relationship? B. Legrand, M. Laurencin, C. Zatylny-Gaudin, J. Henry, A. Bondon and M. Baudy Floc'h -- 1. Introduction -- 2. Results and Discussion -- 3. Conclusion -- References -- Differential antimicrobial activities of Human Beta-Defensins against Methicillin Resistant (MRSA) and Methicillin sensitive (MSSA) Staphylococcus aureus N. D. S. Herathge, J. T. George and D. A. Rowley -- Introduction -- Materials and Methods -- Results -- Discussion -- References -- Isolation of antimicrobial peptides from New Zealand spinach Tetragonia tetragonioides T. Neubauerová, M. Macková, T. Macek, M. Šanda, M. Králová, I. Doležílková and P. N. Holmsgaard -- 1. General remarks -- 2. Materials and methods -- 2.1 Extraction of peptides and proteins from spinach leaves -- 2.2 Purification of crude extract -- 2.3 Reverse-phase chromatography purification (RP-HPLC) -- 2.4 Microorganisms and antimicrobial assay -- 2.5 Antimicrobial activity -- 3. Results -- 3.1 Isolation of antimicrobial peptides from spinach -- 3.2 Antimicrobial assay -- 3.3 Characterization by MS -- 4. Discussion -- References -- Lysostaphin: molecular changes that preserve staphylolytic activity S. Becker, J. Foster-Frey, A. Powell, H. Mohammadi, D. E. Kerr and D. M. Donovan -- 1. Introduction
• Increased resistance to detergent in Enterococcus faecalis Jacqueline Keyhani and Ezzatollah Keyhani -- 1. Introduction -- 2. Materials and Methods -- 3. Results -- 3.1 Comparative sensitivity to detergents of E. faecalis and E. coli -- 3.2 Emergence of an E. faecalis mutant strain with accrued resistance to sarkosyl -- 3.3 Further characteristics of the E. faecalis mutant strain -- 3.4 Antibiotics resistance in the E. faecalis mutant strain -- 4. Discussion -- References -- Legionella pneumophila isolation rate in a Spanish hospital pre- and post-installation of an electrochemical activation system for potable water disinfection Jose-Maria Rivera, Juan-Jose Granizo, Jose-Maria Aguiar, Ana Vos-Arenilla, Maria-Jose Giménez and Lorenzo Aguilar -- INTRODUCTION -- MATERIALS AND METHODS -- RESULTS AND DISCUSSION -- REFERENCES -- Antimicrobial Evaluation: Clinical and Pre-clinical Trials -- Adherence to ART and its associated factors among HIV Aids Patients in Addis Ababa Ezra Muluneh -- 1. Introduction -- 2. Methods -- 3. Result -- 3.1 Background characteristics -- 3.1.1 Spiritual characteristics -- 3.1.2 Factors associated with adherence -- 3.1.2.1 Demographic factors -- 3.1.2.2 Socio-cultural factors -- 3.2 Discussion -- 3.2.1 Level of Adherence -- 3.2.2 Factors associated with adherence -- 3.2.2.1 Demographic factors -- 3.2.2.2 Socio-cultural factors -- 3.3 Limitations of the study -- 3.4 Conclusion and Recommendation -- REFERENCE -- Effectiveness and safety of miconazole with hydrocortisone (Daktacort) feminine care cream in the treatment of vulvar candidiasis J. Perez-Peralta and G. Balaccua -- 1. Introduction -- 2. Study Objectives -- 3. Methods -- 3.1 Study Design -- 3.2 Inclusion and Exclusion Criteria -- 3.3 Treatment and Evaluations -- 3.4 Evaluation Criteria -- 4. Results -- 4.1 Patient Profile -- 4.2 Concomitant Medications and Diseases