Structural analysis of d(GCAATTGC) sub 2 and its complex with berenil by nuclear magnetic resonance spectroscopy

• Published in: Biochemistry (Easton) Vol. 29:28
• Main Authors: Yoshida, Mitsuru, Banville, D.L., Shafer, R.H.
• Format: Journal Article
• Language: English
• Published: United States 17.07.1990
• Subjects: 550201 - Biochemistry- Tracer Techniques; ADENINES; AMIDINES; AMINES; ANTIMETABOLITES; AROMATICS; AZAARENES; BARYONS; BASIC BIOLOGICAL SCIENCES; CHEMICAL SHIFT; DNA; DRUGS; ELEMENTARY PARTICLES; FERMIONS; HADRONS; HETEROCYCLIC COMPOUNDS; MAGNETIC RESONANCE; MATHEMATICAL MODELS; MOLECULAR MODELS; NUCLEAR MAGNETIC RESONANCE; NUCLEIC ACID DENATURATION; NUCLEIC ACIDS; NUCLEONS; OLIGONUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; OVERHAUSER EFFECT; PROTONS; PURINES; RESONANCE; TRYPANOSOMES

The structures of d(GCAATTGC){sub 2} and its complex with berenil in solution were analyzed by two-dimensional {sup 1}H NMR spectroscopy. Intra- and internucleotide nuclear Overhauser effect (NOE) connectivities demonstrate that the octanucleotide duplex is primarily in the B conformation. Binding w...