Three-dimensional heteronuclear NMR of sup 15 N-labeled proteins

The use of a 3D NMR technique has been shown to remove the problems of degenerate chemical shifts. A very sensitive 3D experiment for unraveling the regular protein NOESY spectrum is reported here. The method involves the {sup 15}N labelling of the protein and is described in some detail. The sensit...

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Published inJournal of the American Chemical Society Vol. 111:4
Main Authors Marion, D., Kay, L.E., Sparks, S.W., Torchia, D.A., Bax, A.
Format Journal Article
LanguageEnglish
Published United States 15.02.1989
Subjects
550201 - Biochemistry- Tracer Techniques
BASIC BIOLOGICAL SCIENCES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAGNETIC RESONANCE
MOLECULAR STRUCTURE
NITROGEN 15
NITROGEN ISOTOPES
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RESONANCE
STABLE ISOTOPES
TRACER TECHNIQUES
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Abstract The use of a 3D NMR technique has been shown to remove the problems of degenerate chemical shifts. A very sensitive 3D experiment for unraveling the regular protein NOESY spectrum is reported here. The method involves the {sup 15}N labelling of the protein and is described in some detail. The sensitivity of this heteronuclear 3D technique is excellent, and resonance overlap in the S. Nase 3D spectrum is minimal, despite the relatively coarse digitization. 14 references, 2 figures.
AbstractList The use of a 3D NMR technique has been shown to remove the problems of degenerate chemical shifts. A very sensitive 3D experiment for unraveling the regular protein NOESY spectrum is reported here. The method involves the {sup 15}N labelling of the protein and is described in some detail. The sensitivity of this heteronuclear 3D technique is excellent, and resonance overlap in the S. Nase 3D spectrum is minimal, despite the relatively coarse digitization. 14 references, 2 figures.
Author Torchia, D.A.
Sparks, S.W.
Kay, L.E.
Bax, A.
Marion, D.
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  fullname: Bax, A.
  organization: National Institutes of Health, Bethesda, MD (USA)
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SubjectTerms 550201 - Biochemistry- Tracer Techniques
BASIC BIOLOGICAL SCIENCES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAGNETIC RESONANCE
MOLECULAR STRUCTURE
NITROGEN 15
NITROGEN ISOTOPES
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RESONANCE
STABLE ISOTOPES
TRACER TECHNIQUES
Title Three-dimensional heteronuclear NMR of sup 15 N-labeled proteins
URI https://www.osti.gov/biblio/5031230
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