Monitoring the compaction of single DNA molecules in Xenopus egg extract in real time

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 120; no. 12
• Main Authors: Sun, Mingxuan, Amiri, Hossein, Tong, Alexander B., Shintomi, Keishi, Hirano, Tatsuya, Bustamante, Carlos, Heald, Rebecca
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 14.03.2023
• Subjects: Science & Technology - Other Topics

DNA compaction is required for the condensation and resolution of chromosomes during mitosis, but the relative contribution of individual chromatin factors to this process is poorly understood. We developed a physiological, cell-free system using high-speedXenopusegg extracts and optical tweezers to...