DYNAMIC MR IMAGING, BIODISTRIBUTION AND PHARMACOKINETICS OF POLYMER SHELLED MICROBUBBLES CONTAINING SPION

• Published in: Nano : brief reports and reviews pp. 136 - 148
• Main Authors: ÅSA BARREFELT, MOUSTAPHA HASSAN, HEBA ASEM, PETER ASPELIN, TORKEL B. BRISMAR, GAIO PARADOSSI, SILVIA MARGHERITELLI, MARYAM SAGHAFIAN, LETIZIA ODDO, MAMOUN MUHAMMED
• Format: Journal Article
• Language: English
• Published: 성균나노과학기술원 01.08.2014
• Subjects: 자연과학
• ISSN: 1793-2920

Magnetic Resonance Imaging (MRI) is a noninvasive diagnostic method that provides informa-tion on morphological and physiological changes of the internal organs over time. Imaging andmeasurements can be repeated on the same subject, thereby reducing inter-individual variabilitye®ects and hence the number of subjects required. A potential MRI contrast agent consisting ofmicrobubbles embedded with superparamagnetic iron oxide nanoparticles (SPION) in the shell(SPION MBs) was injected intravenously into rats to determine their biodistribution andpharmacokinetics using MR imaging. Agarose phantoms containing SPION MBs were scannedusing 3 T MRI to construct a standard curve. Rats were injected with SPION MBs, free SPIONor plain MBs and scanned dynamically at 3 T using a clinical MR scanner. The relaxation rate(R2*) was studied over time as a measure of the iron oxide concentrations to enable calculation ofthe pharmacokinetic parameters. The kinetics of SPION MBs in the liver was ¯tted to a one-compartment model. Furthermore, the biological fate of SPION MBs was examined via a his-tological survey of tissue samples using Perls' Prussian blue staining and immunohistochemistry(IHC). 1.2 h after injection of SPION MBs, T2* of the liver had decreased to its minimum. Theelimination half-life of SPION MBs was 598 : 2 97 : 3 h, while the half-life for SPION was222 : 6 26 : 4 h. Moreover, our study showed that SPION MBs were taken up by the macrophagesin the lungs, spleen and liver. MBs labeled with SPION can be used for MR imaging. Moreover,MRI is a reliable and noninvasive tool that can be utilized in pharmacokinetic investigations offuture contrast agents using SPION MBs and SPION in the rat. KCI Citation Count: 0