Heat Stress가 소 난자의 체외성숙과 배반포 발달에 미치는 영향

• Published in: Journal of animal reproduction & biotechnology (Online) pp. 65 - 71
• Main Authors: 김민수, 김성우, 김찬란, 성환후, 김남태
• Format: Journal Article
• Language: Korean
• Published: 사단법인 한국동물생명공학회 01.09.2017
• Subjects: 축산학
• ISSN: 2671-4639; 2671-4663

The elevated temperature and high humidity has been known as main reason for heat stress on animals and cause detrimental effects on productivity of organisms and physiological conditions of normal bioactivities. The aims of this study were to evaluate the relationship between time of heat shock simulation during in vitro maturation and developmental competence of subsequent embryo after in vitro fertilization. Heat shocked cumulus-oocyte complexes (COCs) of Korean native cattle were subjected to normal conditions for 22, 21, 18 and 12 h respectively and transferred to heat stress inducing condition at 40.5 ℃ in other incubator for 0 (control), 1 and 4 h. After maturation for 22 h, the oocytes were fertilized and cultured in mSOF media for 8 d and examined the developmental capacity of embryos. There were no differences in maturation and cleavage rates between 0, 1 and 4 h heat socked oocytes, but blastocysts formation were lower in the 4 h heat stressed oocytes. The apoptotic cells of developed blastocysts were also increased in at day 8 with 4 h heat shocked oocytes. These results indicate that heat shock on oocytes during maturation could cause negative effects on the developmental competence of embryos. KCI Citation Count: 0