Quantitative Changes of PR Proteins and Antioxidative Enzymes in Responseto Glomus intraradices and Phytophthora capsici in Pepper (Capsicum annuumL.) Plants

• Published in: Journal of microbiology and biotechnology pp. 553 - 562
• Main Authors: ZHENG, HU-ZHE, 김용웅, HYUN-JIN LEE, 박노동, WOO-JIN JUNG, 김영철, SANG-HYUN LEE, 김태환, 김길용
• Format: Journal Article
• Language: English
• Published: 한국미생물·생명공학회 01.06.2004
• Subjects: 생물학
• ISSN: 1017-7825

To investigate protective activity in pepper plants,which were pre-inoculated with arbuscular mycorrhizal (AM)fungi Glomus intraradices (Gi), against pathogenic strainPhytophthora capsici (Pc), pathogenesis-related (PR) proteinsand antioxidant enzymes were examined. The growth of rootand shoot was the highest in peppers inoculated with G.intraradices, compared with non-inoculated control plants andthose challenged by the pathogen with and without mycorrhizaeafter nine days of infection. Mycorrhizal colonization ratewas reduced by about 10% in pathogen-challenged plants, butdisease pressure was reduced. The activities of PR proteins,b-1-3-glucanase and chitinase, were increased in Pc-treatedplants compared to Gi+Pc-treated plants in leaves, but thosein roots were suppressed. Superoxide dismutase activity andH2O2 content in Gi+Pc and Pc-treated plants were graduallyincreased in leaves. However, those in roots continuouslyincreased up to 5 days, and then decreased dramatically.Peroxidase activity in leaves and roots increased after P.capsici infection both in plants inoculated with or withoutG. intraradices. These results suggest that AM fungi,G. intraradices, potentially act as one of the protectiveagents against plant pathogens. Changes of PR proteinsand antioxidative enzymes in mycorrhizae-inoculated pepperappear to be regulated differently in leaves and roots bypathogen infection. KCI Citation Count: 8