Cloning of Human Liver Cytosolic Sialidase from Genomic DNA UsingSplicing by Overlap Extension and Its Characterization

Cytosolic sialidase (Neu2), a member of thesialidase family that is responsible for hydrolysis of sialic acidfrom the terminal position of sialoglycoconjugates, is poorlyexpressed in skeletal muscle and not detected in any otheradult tissues. Thus, we isolated Neu2 cDNA using splicing byoverlap exte...

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Bibliographic Details
Published inJournal of microbiology and biotechnology pp. 722 - 727
Main Authors HA, KI-TAE, SEUNG-HAK CHO, SUNG-KOO KANG, YEON-KYE KIM, JUNE-KI KIM, CHEORL-HO KIM
Format Journal Article
LanguageKorean
Published 한국미생물·생명공학회 01.08.2005
Subjects
생물학
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