Cloning and Characterization of Squalene Synthase (SQS) Gene fromGanoderma lucidum

• Published in: Journal of microbiology and biotechnology pp. 1106 - 1112
• Main Authors: ZHAO, MING-WEN, WAN-QI LIANG, DA-BING ZHANG, NAN WANG, CHEN-GUANG WANG, YING-JIE PAN
• Format: Journal Article
• Language: Korean
• Published: 한국미생물·생명공학회 01.07.2007
• Subjects: 생물학
• ISSN: 1017-7825

This report provides the complete nucleotidesequences of the full-length cDNA encoding squalene synthase(SQS) and its genomic DNA sequence from a triterpene-producingfungus, Ganoderma lucidum. The cDNA of the squalenesynthase (SQS) (GenBank Accesion Number: DQ494674)was found to contain an open reading frame (ORF) of 1,404 bpencoding a 468-amino-acid polypeptide, whereas the SQS genomicDNA sequence (GenBank Acesion Number: DQ494675)consisted of 1,984 bp and contained four exons and threG. lucidumgenome. The deduced amino acid sequence of Ganodermalucidum squalene synthase (Gl-SQS) exhibited a high homologywith other fungal squalene synthase genes and contained sixconserved domains. A phylogenetic analysis revealed that G.lucidum SQS belonged to the fungi SQS group, and was moreclosely related to the SQS of U. maydis than to those of otherfungi. A gene expression analysis showed that the expressionincreased after 14 to 20 days of incubation, and reached arelatively high level in the mushroom primordia. Functionalcomplementation of Gl-SQS in a SQS-deficient strain ofSaccharomyces cerevisiae confirmed that the cloned cDNAencoded a squalene synthase. KCI Citation Count: 31