Hepatoprotective effects of reynosin against thioacetamideinduced apoptosis in primary hepatocytes and mouse liver

• Published in: Archives of pharmacal research pp. 485 - 494
• Main Authors: Soohyun Lim, 이성진, 남궁우, 김경호, 마응천
• Format: Journal Article
• Language: English
• Published: 대한약학회 01.04.2013
• Subjects: 약학
• ISSN: 0253-6269; 1976-3786

The aim of this study was to identify thehepatoprotective effects of reynosin, sesquiterpenes fromthe leaves of Laurus nobilis, against thioacetamide (TAA)-induced apoptosis in primary hepatocyte cultures and anin vivo mouse model. Rat hepatocytes were isolated andpretreated with 0.13, 0.64, or 3.22 lM reynosin and thenexposed to 100 mM TAA. Reynosin treatment significantlyinhibited TAA-induced apoptosis and hepatocellular DNAdamage in primary rat hepatocytes. We observed anincrease in levels of antiapoptotic Bcl-2, Bcl-XL mRNAand a decrease in levels of proapoptotic Bax mRNA followingreynosin treatment of hepatocytes. Apoptosis inBALB/c mice was induced with intra-peritoneal injectionof 200 mg/kg TAA for 2 weeks every other day. Thenreynosin (5 mg/kg) and TAA were intragastrically givenfor 3 weeks every other day. Aspartate aminotransferaseand alanine aminotransferase levels in the blood of micewere decreased in the reynosin administration group. Bcl-2and Bcl-XL mRNA levels were increased, and the BaxmRNA level was decreased in reynosin-treated mice. Thus,reynosin inhibited TAA-induced apoptosis in primaryhepatocytes and an in vivo mouse model. KCI Citation Count: 20