Development and Testing of an Individual Kernel Detection System for Genetically Modified Soybean Events in Non-identity-preserved Soybean Samples

• Published in: Biological and Pharmaceutical Bulletin Vol. 43; no. 8; pp. 1259 - 1266
• Main Authors: Keisuke Sogaa, Shinya Kimataa, Jumpei Narushimaa, Sakiko Satob, Emi Satob, Junichi Manoc, Reona Takabatakec, Kazumi Kittac, Hiroshi Kawakamib, Hiroshi Akiyamaa, Kazunari Kondoa, Kosuke Nakamuraa
• Format: Journal Article
• Language: Japanese
• Published: Pharmaceutical Society of Japan 01.08.2020
• ISSN: 0918-6158; 1347-5215

A genetically modified (GM) soybean kernel detection system using combination of DNA preparation from individual soybean kernels and event-specific real-time PCR was developed to simultaneously identify GM soybean events authorized for food after safety assessments in Japan. Over 100 kernels in the non-identity-preserved soybean samples imported from the United States of America (two U.S.A. lots) and Brazil (one lot) were randomly selected and examined. In total, 98 and 96% of the two independent U.S.A. lots, and 100% of the Brazilian lot contained GM soybean kernels. Herbicide-tolerant events, MON89788 (trade name Genuity(R) Roundup Ready 2 Yield(TM)), GTS 40-3-2 (trade name Roundup Ready(TM) soybean) and A2704-12 (trade name Liberty Link(R) soybean), were detected similarly in both U.S.A. lots. In the Brazilian lot, in addition to GTS 40-3-2, a stacked GM event, MON87701×MON89788, having insect-resistance and herbicide-tolerance, was detected. There were no unauthorized GM soybeans comingled, and the ratio of GM soybean events detected was consistent with statistical reports on the cultivated GM soybean events in both countries.