Complete genome and phylogenetic analysis of bovine papillomavirus type 15 in Southern Xinjiang dairy cow
Background: Bovine papilloma is a neoplastic disease caused by bovine papillomaviruses (BPVs), which were recently divided into 5 genera and at least 24 genotypes. Objectives: The complete genome sequence of BPV type 15 (BPV Aks-02), a novel putative BPV type from skin samples from infected cows in...
Saved in:
Published in | Journal of veterinary science (Suwŏn-si, Korea) Vol. 21; no. 6; p. 73.1 |
---|---|
Main Authors | , , , , , , , , |
Format | Journal Article |
Language | Korean |
Published |
2020
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Background: Bovine papilloma is a neoplastic disease caused by bovine papillomaviruses (BPVs), which were recently divided into 5 genera and at least 24 genotypes. Objectives: The complete genome sequence of BPV type 15 (BPV Aks-02), a novel putative BPV type from skin samples from infected cows in Southern Xinjiang China, was determined by collecting warty lesions, followed by DNA extraction and amplicon sequencing. Methods: DNA was analyzed initially by polymerase chain reaction (PCR) using the degenerate primers FAP59 and FAP64. The complete genome sequences of the BPV Aks-02 were amplified by PCR using the amplification primers and sequencing primers. Sequence analysis and phylogenetic analysis were performed using bio-informatic software. Results: The nucleotide sequence of the L1 open reading frame (ORF) of BPV Aks-02 was 75% identity to the L1 ORF of BPV-9 reference strain from GenBank. The complete genome consisted of 7,189 base pairs (G + C content of 42.50%) that encoded 5 early (E8, E7, E1, E2, and E4) and 2 late (L1 and L2) genes. The E7 protein contained a consensus CX2CX29CX2C zinc-binding domain and a LxCxE motif. Among the different members of this group, the percentages of the complete genome and ORFs (including 5 early and 2 late ORFs) sequence identity of BPV Aks-02 were closer to the genus Xipapillomavirus 1 of the Xipapillomavirus genus. Phylogenetic analysis and sequence similarities based on the L1 ORF of BPV Aks-02 revealed the same cluster. Conclusions: The results suggest that BPV type (BPV Aks-02) clustered with members of the Xipapillomavirus genus as BPV 15 and were closely related to Xipapillomavirus 1. |
---|---|
AbstractList | Background: Bovine papilloma is a neoplastic disease caused by bovine papillomaviruses (BPVs), which were recently divided into 5 genera and at least 24 genotypes. Objectives: The complete genome sequence of BPV type 15 (BPV Aks-02), a novel putative BPV type from skin samples from infected cows in Southern Xinjiang China, was determined by collecting warty lesions, followed by DNA extraction and amplicon sequencing. Methods: DNA was analyzed initially by polymerase chain reaction (PCR) using the degenerate primers FAP59 and FAP64. The complete genome sequences of the BPV Aks-02 were amplified by PCR using the amplification primers and sequencing primers. Sequence analysis and phylogenetic analysis were performed using bio-informatic software. Results: The nucleotide sequence of the L1 open reading frame (ORF) of BPV Aks-02 was 75% identity to the L1 ORF of BPV-9 reference strain from GenBank. The complete genome consisted of 7,189 base pairs (G + C content of 42.50%) that encoded 5 early (E8, E7, E1, E2, and E4) and 2 late (L1 and L2) genes. The E7 protein contained a consensus CX2CX29CX2C zinc-binding domain and a LxCxE motif. Among the different members of this group, the percentages of the complete genome and ORFs (including 5 early and 2 late ORFs) sequence identity of BPV Aks-02 were closer to the genus Xipapillomavirus 1 of the Xipapillomavirus genus. Phylogenetic analysis and sequence similarities based on the L1 ORF of BPV Aks-02 revealed the same cluster. Conclusions: The results suggest that BPV type (BPV Aks-02) clustered with members of the Xipapillomavirus genus as BPV 15 and were closely related to Xipapillomavirus 1. |
Author | Song, Yumeng Zhang, Yingyu Hu, Jianjun Wang, Zhehong Cheng, Long Zhang, Wanqi Zhao, Yubing Chauhan, Surinder Singh Shi, Changqing |
Author_xml | – sequence: 1 fullname: Hu, Jianjun – sequence: 2 fullname: Zhang, Wanqi – sequence: 3 fullname: Chauhan, Surinder Singh – sequence: 4 fullname: Shi, Changqing – sequence: 5 fullname: Song, Yumeng – sequence: 6 fullname: Zhao, Yubing – sequence: 7 fullname: Wang, Zhehong – sequence: 8 fullname: Cheng, Long – sequence: 9 fullname: Zhang, Yingyu |
BookMark | eNqNjrFuwkAQRE8RSIGEf9iG0pKxffa5jBBRlBQUSeEOHfYCS867J99B5L-Pi3xAqpk3esUs1YyF8UEtNnVVJlrrZjb1LKsTU-jmUa1CoGOq87wqTWUWirbSe4cR4YwsPYLlDvxldDIxRmqnwboxUAA5wVHuxAjeenJOenun4RYgjh5ho4EYPuUWLzgwNMRXsnyGztIwQis_z2p-si7g6i-f1Pp197V9S74pRDpwF9zh_eVjn6VZOh3WpkzrojL5f71fj2pK4Q |
ContentType | Journal Article |
DBID | JDI |
DEWEY | 636.089 |
DatabaseName | KoreaScience |
DatabaseTitleList | |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Veterinary Medicine |
DocumentTitleAlternate | Complete genome and phylogenetic analysis of bovine papillomavirus type 15 in Southern Xinjiang dairy cow |
EISSN | 1976-555X |
EndPage | 73.10 |
ExternalDocumentID | JAKO202012258609478 |
GroupedDBID | --- .3O 29L 2WC 36B 5-W 53G 5GY 5VS 85H 8JR 8XY 9ZL AAFWJ ACGFO ADBBV ADRAZ AEGXH AENEX AFPKN AIAGR ALMA_UNASSIGNED_HOLDINGS AOIJS BAWUL BCNDV C1A C~G DBRKI DIK DYU E3Z EBD EBS ECGQY EF. EJD EMB EMOBN EYRJQ F5P GROUPED_DOAJ HYE ITG ITH JDI KQ8 M48 MZR M~E N.T OK1 OZF P2P P5Y RNS RPM SV3 TDB TR2 XSB ZZE ~KM |
ID | FETCH-kisti_ndsl_JAKO2020122586094783 |
IEDL.DBID | M48 |
ISSN | 1229-845X |
IngestDate | Fri Dec 22 12:04:04 EST 2023 |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 6 |
Keywords | Bovine papillomavirus Xipapillomavirus Phylogenetics Sequencing |
Language | Korean |
LinkModel | DirectLink |
MergedId | FETCHMERGED-kisti_ndsl_JAKO2020122586094783 |
Notes | KISTI1.1003/JNL.JAKO202012258609478 |
OpenAccessLink | http://journals.scholarsportal.info/openUrl.xqy?issn=1229-845X&volume=21&issue=6&spage=73.1 |
ParticipantIDs | kisti_ndsl_JAKO202012258609478 |
PublicationCentury | 2000 |
PublicationDate | 2020 |
PublicationDateYYYYMMDD | 2020-01-01 |
PublicationDate_xml | – year: 2020 text: 2020 |
PublicationDecade | 2020 |
PublicationTitle | Journal of veterinary science (Suwŏn-si, Korea) |
PublicationTitleAlternate | Journal of veterinary science |
PublicationYear | 2020 |
SSID | ssib053376878 ssib044746103 ssj0032569 |
Score | 4.425688 |
Snippet | Background: Bovine papilloma is a neoplastic disease caused by bovine papillomaviruses (BPVs), which were recently divided into 5 genera and at least 24... |
SourceID | kisti |
SourceType | Open Access Repository |
StartPage | 73.1 |
Title | Complete genome and phylogenetic analysis of bovine papillomavirus type 15 in Southern Xinjiang dairy cow |
URI | http://click.ndsl.kr/servlet/LinkingDetailView?cn=JAKO202012258609478&dbt=JAKO&org_code=O481&site_code=SS1481&service_code=01 |
Volume | 21 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwhV07T8MwELaqTiwICohndQN0S0Ud5-EJVahVVVRYKMpW2YmDDMEpactj4bdzTkJhQLBksJJc4rvP993ZPhNyKqWXsN45d0KhpMOoCBypUumELI5jxRMWlNvFJtf-aMrGkRc1yNf6-boDF7-GdvY8qWmRdd-e3y8qtFKKQpgXdWoo015H15is8Bi43TI0oq61_sk39WcssJXG17aJrAeJd7CegXCRCljqvBaB5NUyOv3D-wy3yGZNG6Ff6XmbNB7zFmnd2bUs5YZamNRz5DtEW4ijNhTY-qtPCoRJADsTBzkc1_B5bKgKkUCegrQZBQVzMddZlj-JF12sFmATs9DzQBsoz9hThYFImwe0pXtIhEaBcf66S86Gg9vLkVN-8swki2w27l_dUCSH-Ede6GNUF4TuHmma3Kh9Ar6KOYtp4POUszBOBRc0SdH_S5f7PBEHpP33uw7_u-GIbNiWKn1xTJrLYqVO0KEvZbvUjL1-DD4BslOjYw |
link.rule.ids | 230,315,786,790,891,2236,4043 |
linkProvider | Scholars Portal |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Complete+genome+and+phylogenetic+analysis+of+bovine+papillomavirus+type+15+in+Southern+Xinjiang+dairy+cow&rft.jtitle=Journal+of+veterinary+science+%28Suw%C5%8Fn-si%2C+Korea%29&rft.au=Hu%2C+Jianjun&rft.au=Zhang%2C+Wanqi&rft.au=Chauhan%2C+Surinder+Singh&rft.au=Shi%2C+Changqing&rft.date=2020&rft.issn=1229-845X&rft.eissn=1976-555X&rft.volume=21&rft.issue=6&rft.spage=73.1&rft.epage=73.10&rft.externalDBID=n%2Fa&rft.externalDocID=JAKO202012258609478 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1229-845X&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1229-845X&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1229-845X&client=summon |