Anticancer activity of CopA3 dimer peptide in human gastric cancer cells

CopA3 is a homodimeric ${\alpha}$-helical peptide derived from coprisin which is a defensin-like antimicrobial peptide that was identified from the dung beetle, Copris tripartitus. CopA3 has been reported to have anticancer activity against leukemia cancer cells. In the present study, we investigate...

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Published inBMB reports Vol. 48; no. 6; pp. 324 - 329
Main Authors Lee, Joon Ha, Kim, In-Woo, Kim, Sang-Hee, Yun, Eun-Young, Nam, Sung-Hee, Ahn, Mi-Young, Kang, Dong-Chul, Hwang, Jae Sam
Format Journal Article
LanguageKorean
Published 2015
Subjects
CopA3
Anticancer activity
Phosphatidylserine
Antimicrobial peptide
Necrosis
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Summary:CopA3 is a homodimeric ${\alpha}$-helical peptide derived from coprisin which is a defensin-like antimicrobial peptide that was identified from the dung beetle, Copris tripartitus. CopA3 has been reported to have anticancer activity against leukemia cancer cells. In the present study, we investigated the anticancer activity of CopA3 in human gastric cancer cells. CopA3 reduced cell viability and it was cytotoxic to gastric cancer cells in the MTS and LDH release assay, respectively. CopA3 was shown to induce necrotic cell death of the gastric cancer cells by flow cytometric analysis and acridine orange/ethidium bromide staining. CopA3-induced cell death was mediated by specific interactions with phosphatidylserine, a membrane component of cancer cells. Taken together, these data indicated that CopA3 mainly caused necrosis of gastric cancer cells, probably through interactions with phosphatidylserine, which suggests the potential utility of CopA3 as a cancer therapeutic. [BMB Reports 2015; 48(6): 324-329]
Bibliography:KISTI1.1003/JNL.JAKO201525960574936
ISSN:1976-6696
1976-670X