Specific and Sensitive Detection of the Pear Scab Fungus Venturia nashicola by SYBR Green Real-Time PCR

A new improved PCR method has been developed for the rapid, reliable, and sensitive detection of Venturia nashicola, a destructive pathogen of scab disease in Japanese pear. The translation elongation factor-1 alpha gene-derived PCR primers specifically amplified a 257-bp-sized DNA band of the targe...

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Published inJournal of microbiology and biotechnology Vol. 25; no. 11; pp. 1782 - 1786
Main Authors Yun, Yeo Hong, Yoon, Seong Kwon, Jung, Jae Sung, Kim, Seong Hwan
Format Journal Article
LanguageKorean
Published 2015
Subjects
Venturia nashicola
translation elongation factor-1 alpha gene
real-time PCR
Pear scab
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Abstract A new improved PCR method has been developed for the rapid, reliable, and sensitive detection of Venturia nashicola, a destructive pathogen of scab disease in Japanese pear. The translation elongation factor-1 alpha gene-derived PCR primers specifically amplified a 257-bp-sized DNA band of the target gene from the genomic DNA of V. nashicola. No amplicon was produced from the genomic DNA of other Venturia spp. and reference fungal species tested. With the high detection limit of 10 fg DNA content, our real-time method could be used for the quarantine inspection and field monitoring of V. nashicola.
AbstractList A new improved PCR method has been developed for the rapid, reliable, and sensitive detection of Venturia nashicola, a destructive pathogen of scab disease in Japanese pear. The translation elongation factor-1 alpha gene-derived PCR primers specifically amplified a 257-bp-sized DNA band of the target gene from the genomic DNA of V. nashicola. No amplicon was produced from the genomic DNA of other Venturia spp. and reference fungal species tested. With the high detection limit of 10 fg DNA content, our real-time method could be used for the quarantine inspection and field monitoring of V. nashicola.
Author Yoon, Seong Kwon
Kim, Seong Hwan
Yun, Yeo Hong
Jung, Jae Sung
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Keywords Venturia nashicola
translation elongation factor-1 alpha gene
real-time PCR
Pear scab
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Title Specific and Sensitive Detection of the Pear Scab Fungus Venturia nashicola by SYBR Green Real-Time PCR
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