Tumour Suppressive Effects of WEE1 Gene Silencing in Breast Cancer Cells
Background: WEE1 is a G2/M checkpoint regulator protein. Various studies have indicated that WEE1 could be a good target for cancer therapy. The main aim of this study was to asssess the tumor suppressive potential of WEE1 silencing in two different breast cancer cell lines, MCF7 which carries the w...
Saved in:
Published in | Asian Pacific journal of cancer prevention : APJCP Vol. 14; no. 11; pp. 6605 - 6611 |
---|---|
Main Authors | , , , , , , , |
Format | Journal Article |
Language | Korean |
Published |
2013
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Background: WEE1 is a G2/M checkpoint regulator protein. Various studies have indicated that WEE1 could be a good target for cancer therapy. The main aim of this study was to asssess the tumor suppressive potential of WEE1 silencing in two different breast cancer cell lines, MCF7 which carries the wild-type p53 and MDA-MB468 which contains a mutant type. Materials and Methods: After WEE1 knockdown with specific shRNAs downstream effects on cell viability and cell cycle progression were determined using MTT and flow cytometry analyses, respectively. Real-time PCR and Western blotting were conducted to assess the effect of WEE1 inhibition on the expression of apoptotic (p53) and anti-apoptotic (Bcl2) factors and also a growth marker (VEGF). Results: The results showed that WEE1 inhibition could cause a significant decrease in the viability of both MCF7 and MDA-MB-468 breast cancer cell lines by more than 50%. Interestingly, DNA content assays showed a significant increase in apoptotic cells following WEE1 silencing. WEE1 inhibition also induced upregulation of the apoptotic marker, p53, in breast cancer cells. A significant decrease in the expression of VEGF and Bcl-2 was observed following WEE1 inhibition in both cell lines. Conclusions: In concordance with previous studies, our data showed that WEE1 inhibition could induce G2 arrest abrogation and consequent cell death in breast cancer cells. Moreover, in this study, the observed interactions between the pro- and anti-apoptotic proteins and decrease in the angiogenesis marker expression confirm the susceptibility to apoptosis and validate the tumor suppressive effect of WEE1 inhibition in breast cancer cells. Interestingly, the levels of the sensitivity to WEE1 silencing in breast cancer cells, MCF7 and MDA-MB468, seem to be in concordance with the level of p53 expression. |
---|---|
AbstractList | Background: WEE1 is a G2/M checkpoint regulator protein. Various studies have indicated that WEE1 could be a good target for cancer therapy. The main aim of this study was to asssess the tumor suppressive potential of WEE1 silencing in two different breast cancer cell lines, MCF7 which carries the wild-type p53 and MDA-MB468 which contains a mutant type. Materials and Methods: After WEE1 knockdown with specific shRNAs downstream effects on cell viability and cell cycle progression were determined using MTT and flow cytometry analyses, respectively. Real-time PCR and Western blotting were conducted to assess the effect of WEE1 inhibition on the expression of apoptotic (p53) and anti-apoptotic (Bcl2) factors and also a growth marker (VEGF). Results: The results showed that WEE1 inhibition could cause a significant decrease in the viability of both MCF7 and MDA-MB-468 breast cancer cell lines by more than 50%. Interestingly, DNA content assays showed a significant increase in apoptotic cells following WEE1 silencing. WEE1 inhibition also induced upregulation of the apoptotic marker, p53, in breast cancer cells. A significant decrease in the expression of VEGF and Bcl-2 was observed following WEE1 inhibition in both cell lines. Conclusions: In concordance with previous studies, our data showed that WEE1 inhibition could induce G2 arrest abrogation and consequent cell death in breast cancer cells. Moreover, in this study, the observed interactions between the pro- and anti-apoptotic proteins and decrease in the angiogenesis marker expression confirm the susceptibility to apoptosis and validate the tumor suppressive effect of WEE1 inhibition in breast cancer cells. Interestingly, the levels of the sensitivity to WEE1 silencing in breast cancer cells, MCF7 and MDA-MB468, seem to be in concordance with the level of p53 expression. |
Author | Habibagahi, Mojtaba Jaberipour, Mansooreh Rosli, Rozita Abdullah, Syahrilnizam Ghiasi, Naghmeh Ghaderi, Abbas Hosseini, Ahmad Yusoff, Khatijah |
Author_xml | – sequence: 1 fullname: Ghiasi, Naghmeh – sequence: 2 fullname: Habibagahi, Mojtaba – sequence: 3 fullname: Rosli, Rozita – sequence: 4 fullname: Ghaderi, Abbas – sequence: 5 fullname: Yusoff, Khatijah – sequence: 6 fullname: Hosseini, Ahmad – sequence: 7 fullname: Abdullah, Syahrilnizam – sequence: 8 fullname: Jaberipour, Mansooreh |
BookMark | eNqNyrEOgjAUQNHGYCIq__AWR5KWloKjEtTo4ACJbgTxYRprIS34_Tr4AU53OHdOPNMZnBA_EokMExldPeKzmPEw4TKdkcA5daNCJJxKIXxyKMdXN1ooxr63-MU3Qt622AwOuhYuec5gjwahUBpNo8wDlIGtxdoNkNWmQQsZau2WZNrW2mHw64KsdnmZHcKncoOqzN3p6rg5nSPKOI3XKeM8lTHl_34fcH882w |
ContentType | Journal Article |
DBID | JDI |
DEWEY | 616.994 |
DatabaseName | KoreaScience |
DatabaseTitleList | |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Medicine |
DocumentTitleAlternate | Tumour Suppressive Effects of WEE1 Gene Silencing in Breast Cancer Cells |
EISSN | 2476-762X |
EndPage | 6611 |
ExternalDocumentID | JAKO201305981338650 |
GroupedDBID | --- .UV 23N 2WC 53G 5GY 9ZL ADBBV ALMA_UNASSIGNED_HOLDINGS AOIJS BAWUL BCNDV C1A DIK E3Z EBS EJD F5P FRJ GROUPED_DOAJ GX1 HYE JDI OK1 RPM TR2 W2D WOW |
ID | FETCH-kisti_ndsl_JAKO2013059813386503 |
ISSN | 1513-7368 |
IngestDate | Fri Dec 22 11:59:16 EST 2023 |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 11 |
Keywords | viability inhibition WEE1 shRNA Breast cancer G2 arrest abrogation |
Language | Korean |
LinkModel | OpenURL |
MergedId | FETCHMERGED-kisti_ndsl_JAKO2013059813386503 |
Notes | KISTI1.1003/JNL.JAKO201305981338650 |
OpenAccessLink | http://click.ndsl.kr/servlet/LinkingDetailView?cn=JAKO201305981338650&dbt=JAKO&org_code=O481&site_code=SS1481&service_code=01 |
ParticipantIDs | kisti_ndsl_JAKO201305981338650 |
PublicationCentury | 2000 |
PublicationDate | 2013 |
PublicationDateYYYYMMDD | 2013-01-01 |
PublicationDate_xml | – year: 2013 text: 2013 |
PublicationDecade | 2010 |
PublicationTitle | Asian Pacific journal of cancer prevention : APJCP |
PublicationTitleAlternate | Asian Pacific journal of cancer prevention : APJCP |
PublicationYear | 2013 |
SSID | ssib044730644 ssib007832521 ssj0045875 |
Score | 3.9688632 |
Snippet | Background: WEE1 is a G2/M checkpoint regulator protein. Various studies have indicated that WEE1 could be a good target for cancer therapy. The main aim of... |
SourceID | kisti |
SourceType | Open Access Repository |
StartPage | 6605 |
Title | Tumour Suppressive Effects of WEE1 Gene Silencing in Breast Cancer Cells |
URI | http://click.ndsl.kr/servlet/LinkingDetailView?cn=JAKO201305981338650&dbt=JAKO&org_code=O481&site_code=SS1481&service_code=01 |
Volume | 14 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3PT9swFLYQh4nLtA3Q2EblA5yiIJI4TncsVbeq0wCxInpDduzSQJdMbcqhfz3v2WniThUbXKLKba2k79P3fvR9z4QchVKFiVRffR1GzGdxInwRp8xXiRISPJSMbJfvOe9fs8EoHjWlbKMuKeVJutyoK3mNVWEN7Ioq2RdYtt4UFuA12BeuYGG4_p-NF5C4zzw8mdO0sz5qr9f0Z9z0eoEZK-39ylBaVKlXzrANvfS6aO6Z19XT6dyNUDtGVVn16rmDJVL7hT_VzCdEDRYVLwfdWiL2fZKJeWZJ-27yW08afpOZFHfCnCAMPHJfCln7g6tibjXaV8UyK0WzGTZZmzc6Uoq1-oQVlq7INIj8JLLH5pxosxayhPtAwKM1BmYu0gKHTzk_jR3fDMFE0Diu1Z_1f_mzustw0PlxgXcEEWQbU3Fuajs4TshJvZF3EqA1J4xhLMG8jK08OovbZmBz_TyQyGB0nzmRyPAdeVulELRj8fCebD0UH8ibn1WTxC7pW1hQBxa0ggUtxhRhQREWtIYFzXJqYUEtLKiBxR45_tYbdvu-uY3bXM2ntxueNton23mR64-Eaq5TwdNAhWPNJHg5pcdRCtm85Iqr0_iAtJ7f69O_PvCZ7ITmqBAsT30h2-VsoQ8hYCtly_zaT9FjQSU |
link.rule.ids | 230,315,786,790,891,4043 |
linkProvider | Directory of Open Access Journals |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Tumour+Suppressive+Effects+of+WEE1+Gene+Silencing+in+Breast+Cancer+Cells&rft.jtitle=Asian+Pacific+journal+of+cancer+prevention+%3A+APJCP&rft.au=Ghiasi%2C+Naghmeh&rft.au=Habibagahi%2C+Mojtaba&rft.au=Rosli%2C+Rozita&rft.au=Ghaderi%2C+Abbas&rft.date=2013&rft.issn=1513-7368&rft.eissn=2476-762X&rft.volume=14&rft.issue=11&rft.spage=6605&rft.epage=6611&rft.externalDBID=n%2Fa&rft.externalDocID=JAKO201305981338650 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1513-7368&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1513-7368&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1513-7368&client=summon |