Protection of Rabbit Kidney from Ischemia/Reperfusion Injury by Green Tea Polyphenol Pretreatment

Reactive oxygen species (ROS) have been implicated in the pathogenesis of renal injury after ischemia/reperfusion (I/R). Recently, green tea polyphenols (GTP) have been found to protect the myocardium and liver against I/R injury. Less attention, however, has been paid to the protective effects of G...

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Published inArchives of pharmacal research Vol. 30; no. 11; pp. 1447 - 1454
Main Authors Rah, Dong-Kyun, Han, Dong-Wook, Baek, Hyun-Sook, Hyon, Suong-Hyu, Park, Beyoung-Yun, Park, Jong-Chul
Format Journal Article
LanguageKorean
Published 2007
Subjects
Reactive oxygen species
Renal transplantation
Green tea polyphenol
Ischemia/reperfusion
Kidney
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Summary:Reactive oxygen species (ROS) have been implicated in the pathogenesis of renal injury after ischemia/reperfusion (I/R). Recently, green tea polyphenols (GTP) have been found to protect the myocardium and liver against I/R injury. Less attention, however, has been paid to the protective effects of GTP with respect to the kidneys. This study was designed to determine whether GTP could protect renal cells from ischemic injury. The rabbits were divided into three groups of equal size: control (sham-operated), I/R + vehicle (normal saline) and I/R + GTP groups. Each group consisted of six rabbits. Animals underwent 30, 60, 90 and 120 min of ischemia, followed by 24 h of reperfusion, respectively. GTP $(200{\mu}g/kg)$ or the vehicle was administered 45 min prior to commencement of I/R. The results demonstrated that GTP administration resulted in a significant (P < 0.05) reduction of renal damage after 90 min of ischemia, as indicated by the decreased levels of creatinine and urea nitrogen in serum. These results were confirmed by histological examinations, which showed that GTP pretreatment inhibited necrosis and sloughing of the proximal tubules induced by I/R. Examinations also showed decreased necrotic areas in the medulla and decreased glomerular collapse in the I/R-injured rabbits. Moreover, the infiltration of $CD8^+$ T cells was considerably decreased in GTP-treated kidneys. The results of this study suggest that GTP can reduce renal injury by preventing the oxidative stress dependent on I/R and may be used in renal transplantation as an antioxidant.
Bibliography:KISTI1.1003/JNL.JAKO200708410615507
ISSN:0253-6269
1976-3786