Low Molecular Weight Collagen Peptide (LMWCP) Promotes Hair Growth by Activating the Wnt/GSK-3β/β-Catenin Signaling Pathway
Low molecular weight collagen peptide (LMWCP) is a collagen hydrolysate derived from fish. We investigated the effects of LMWCP on hair growth using human dermal papilla cells (hDPCs), human hair follicles (hHFs), patch assay, and telogenic C57BL/6 mice, while also examining the underlying mechanism...
Saved in:
Published in | Journal of microbiology and biotechnology Vol. 34; no. 1; pp. 17 - 28 |
---|---|
Main Authors | , , , , , , , , |
Format | Journal Article |
Language | Korean |
Published |
한국미생물생명공학회
31.01.2024
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Low molecular weight collagen peptide (LMWCP) is a collagen hydrolysate derived from fish. We investigated the effects of LMWCP on hair growth using human dermal papilla cells (hDPCs), human hair follicles (hHFs), patch assay, and telogenic C57BL/6 mice, while also examining the underlying mechanisms of its action. LMWCP promoted proliferation and mitochondrial potential, and the secretion of hair growth-related factors, such as EGF, HB-EGF, FGF-4, and FGF-6 in hDPCs. Patch assay showed that LMWCP increased the neogeneration of new HFs in a dose-dependent manner. This result correlated with an increase in the expression of dermal papilla (DP) signature genes such as, ALPL, SHH, FGF7, and BMP-2. LMWCP upregulated phosphorylation of glycogen synthase kinase-3β (GSK-3β) and β-catenin, and nuclear translocation of β-catenin, and it increased the expression of Wnt3a, LEF1, VEGF, ALP, and β-catenin. LMWCP promoted the growth of hHFs and increased the expression of β-catenin and VEGF. Oral administration of LMWCP to mice significantly stimulated hair growth. The expression of Wnt3a, β-catenin, PCNA, Cyclin D1, and VEGF was also elevated in the back skin of the mice. Furthermore, LMWCP increased the expression of cytokeratin and Keratin Type I and II. Collectively, these findings demonstrate that LMWCP has the potential to increase hair growth via activating the Wnt/β-catenin signaling pathway. |
---|---|
AbstractList | Low molecular weight collagen peptide (LMWCP) is a collagen hydrolysate derived from fish. We investigated the effects of LMWCP on hair growth using human dermal papilla cells (hDPCs), human hair follicles (hHFs), patch assay, and telogenic C57BL/6 mice, while also examining the underlying mechanisms of its action. LMWCP promoted proliferation and mitochondrial potential, and the secretion of hair growth-related factors, such as EGF, HB-EGF, FGF-4, and FGF-6 in hDPCs. Patch assay showed that LMWCP increased the neogeneration of new HFs in a dose-dependent manner. This result correlated with an increase in the expression of dermal papilla (DP) signature genes such as, ALPL, SHH, FGF7, and BMP-2. LMWCP upregulated phosphorylation of glycogen synthase kinase-3β (GSK-3β) and β-catenin, and nuclear translocation of β-catenin, and it increased the expression of Wnt3a, LEF1, VEGF, ALP, and β-catenin. LMWCP promoted the growth of hHFs and increased the expression of β-catenin and VEGF. Oral administration of LMWCP to mice significantly stimulated hair growth. The expression of Wnt3a, β-catenin, PCNA, Cyclin D1, and VEGF was also elevated in the back skin of the mice. Furthermore, LMWCP increased the expression of cytokeratin and Keratin Type I and II. Collectively, these findings demonstrate that LMWCP has the potential to increase hair growth via activating the Wnt/β-catenin signaling pathway. |
Author | Yujin Kim Jung Ok Lee Jung Min Lee Hyeong-min Kim Do-un Kim Mun-hoe Lee Hee-chul Chung Jin-hee Lee Beom Joon Kim |
Author_xml | – sequence: 2 fullname: Yujin Kim – sequence: 3 – sequence: 4 fullname: Jung Ok Lee – sequence: 5 fullname: Jung Min Lee – sequence: 6 fullname: Mun-hoe Lee – sequence: 7 fullname: Hyeong-min Kim – sequence: 8 fullname: Hee-chul Chung – sequence: 9 fullname: Do-un Kim – sequence: 10 fullname: Jin-hee Lee – sequence: 11 fullname: Beom Joon Kim |
BookMark | eNp9yrtqAkEUgOEpFLw-QZpTarE4o9G1DYsXiMKCwpZyYk52TzLOyMxJli3yUj6IzxQCqVP9xff3VMt5Ry3VNdqkSbqczjuqF-O71gszXS666nvna9h7S-dPiwEK4rISyLy1WJKDnK7CrwSj3b7I8jHkwV-8UIQtcoBN8LVU8NLA01n4C4VdCVIRFE4mm8NzMrvfJvdbkqGQYwcHLh3a3ylHqWpsBqr9hjbS8K999bBeHbNt8sExnq6BLxia06NOtTHz2f_6A5BXSkE |
ContentType | Journal Article |
DBID | HZB Q5X |
DatabaseName | KISS = 한국의핵심지식정보자원 Korean Studies Information Service System (KISS) B-Type |
DatabaseTitleList | |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Biology |
DocumentTitleAlternate | Low Molecular Weight Collagen Peptide (LMWCP) Promotes Hair Growth by Activating the Wnt/GSK-3β/β-Catenin Signaling Pathway |
ExternalDocumentID | 4070115 |
GroupedDBID | --- .UV 29L 53G 5GY 85H 9ZL ACYCR ADBBV AENEX ALMA_UNASSIGNED_HOLDINGS BAWUL DIK DU5 F5P FRP GX1 HZB JDI OK1 P2P Q5X RPM SDH TR2 |
ID | FETCH-kiss_primary_40701153 |
ISSN | 1017-7825 |
IngestDate | Wed Feb 07 02:14:21 EST 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 1 |
Language | Korean |
LinkModel | OpenURL |
MergedId | FETCHMERGED-kiss_primary_40701153 |
Notes | The Korean Society for Applied Microbiology |
PageCount | 12 |
ParticipantIDs | kiss_primary_4070115 |
PublicationCentury | 2000 |
PublicationDate | 20240131 |
PublicationDateYYYYMMDD | 2024-01-31 |
PublicationDate_xml | – month: 01 year: 2024 text: 20240131 day: 31 |
PublicationDecade | 2020 |
PublicationTitle | Journal of microbiology and biotechnology |
PublicationTitleAlternate | Journal of Microbiology and Biotechnology |
PublicationYear | 2024 |
Publisher | 한국미생물생명공학회 |
Publisher_xml | – name: 한국미생물생명공학회 |
SSID | ssj0061286 ssib053376806 |
Score | 4.7537127 |
Snippet | Low molecular weight collagen peptide (LMWCP) is a collagen hydrolysate derived from fish. We investigated the effects of LMWCP on hair growth using human... |
SourceID | kiss |
SourceType | Publisher |
StartPage | 17 |
SubjectTerms | hDPCs human dermal papilla cells LMWCP low molecular weight collagen peptide VEGF Wnt3a β-catenin |
Title | Low Molecular Weight Collagen Peptide (LMWCP) Promotes Hair Growth by Activating the Wnt/GSK-3β/β-Catenin Signaling Pathway |
URI | https://kiss.kstudy.com/ExternalLink/Ar?key=4070115 |
Volume | 34 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1fT9swELcK0qS9TPwZ2sYf-WGWNlUZWx036WPipuvWdlSCqewJJcEdBdEiSFWBtM-zdz4In4k72wkp9IHtJbIusRPlfjn_fDnfEfKeJ0IkYBKdGD4Hx_Vd34k_K99Jk7pwVXpcG-oynb0f9fZP9_uhOKxU_pailqZZ8im9Wbiv5H-0CjLQK-6S_QfNFoOCANqgXziChuH4LB13JzP4KG192-pAezm1KwCMBNg2DFg51hyy2xvIPjoA-jr6Tl1V2_HoEh1Ps-wEGWiQmjJndu_UALMRtL7udxzOZMRCTJ9qGo4EcooJWfdHv5HDQ4c-kMhZPPd7uERzz0ePUj1BO3viz_81PYUxO6PzIqIHjFB176zaVWpO1IPLSrLedOycTFQhsv6LGsa85IYfEceiJmsI1pAsClgoWNBkUciCFgt9Fknmc9YwEslCOS8JmC90L44d7TgN3YBTsmTVcSoGKiTKZt_6UMvwNjbc7CV9lIUb1r1InpfIEv-CkaPNb53cbAFhhjUb_qw2BAA4oy4wWtwVZntYDFyVeMvBCnllNUEDg55VUjmbrJEXpgTp9Tr5AxiiBYaowRDNMUQthugHjaCPNMcPRfxQgx-aXNMH_FDADwX87Gr03N3u3t3mmKEFZqjFzGuy2YoOZNvBJz-6MJlPjuxb4BtkeTwZqzeEptyDWWHoebFQ7tDjfgoM001hHlA1XlfeW7K-aIR3i8Wb5OUDRLbIcnY5VdtA-rJkR7_yezKXVZA |
link.rule.ids | 315,786,790 |
linkProvider | Flying Publisher |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Low+Molecular+Weight+Collagen+Peptide+%28LMWCP%29+Promotes+Hair+Growth+by+Activating+the+Wnt%2FGSK-3%CE%B2%2F%CE%B2-Catenin+Signaling+Pathway&rft.jtitle=Journal+of+microbiology+and+biotechnology&rft.au=Yujin+Kim&rft.au=Jung+Ok+Lee&rft.au=Jung+Min+Lee&rft.au=Mun-hoe+Lee&rft.date=2024-01-31&rft.pub=%ED%95%9C%EA%B5%AD%EB%AF%B8%EC%83%9D%EB%AC%BC%EC%83%9D%EB%AA%85%EA%B3%B5%ED%95%99%ED%9A%8C&rft.issn=1017-7825&rft.volume=34&rft.issue=1&rft.spage=17&rft.externalDocID=4070115 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1017-7825&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1017-7825&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1017-7825&client=summon |