위암 조직에서의 암유전자 발현에 관한 연구

• Published in: The Korean journal of gastroenterology Vol. 21; no. 2; pp. 311 - 320
• Main Authors: 이동후, Dong Hoo Lee, 이중달, Jung Dal Lee
• Format: Journal Article
• Language: Korean
• Published: 대한소화기학회 01.01.1989
• ISSN: 1598-9992

The expression of cellular myc (c-myc) was investigated in human primary stomach cancer and gastric mucosal dysplasia employing in situ hybridizaton. Immunohistochemical reaction of avidin- biotin alkaiine phosphatase with substrates was employed to detect a signal of the biotin-labeled c-myc oncogene hybridized in target sections of paraffin-embedded tissues. The c-myc oncogene showed its expression as the fine grains or clumpy clusters of grains in the nuclei or perinuclear cytoplasm, whereas there was no demonstrable colour reaction in control for specificity testing by an excluding the c-myc cDNA probe during the whole procedure. The expression of c-myc was greatly enhanced in seven advanced adenocarcinoma of the stomach with individual and cell-to-cell variation. Early gastric cancer that means adenocarcinoma confined to a gastric mucosa and submucosa in four subjects yield also amplified c-myc expression but less than in advanced cancer. In contrast, both the dysplasia of five patients and the non-neoplastic tissue cells in the vicinity of cancers expressed weak elevation of the c-myc. Our data indicate that c-mvc expression is heterogeneously elevated during the multistep carcinogenesis and the stomach cancers. Furthermore, the data support that dysplaia or atypical hyperplasia is a potential precancerous lesion of stomach cancer