The Radiation-Sensitive Costimulatory Factors Involved in B-Cell-Dependent T-Cell Activation by Minor Lymphocyte Stimulating Antigen
The regulation of CD28/B7 is important in T-cell activation. It has been argued that its aberrant expression is involved in the radiosensitivity of B-cell-stimulated T-cell response. Here, this possibility is studied in the mixed lymphocyte reaction (MLR) induced by minor lymphocyte-stimulating (Mls...
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| Published in | Journal of biomedical science Vol. 5; no. 5; pp. 332 - 342 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
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Basel, Switzerland
01.05.1998
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| Abstract | The regulation of CD28/B7 is important in T-cell activation. It has been argued that its aberrant expression is involved in the radiosensitivity of B-cell-stimulated T-cell response. Here, this possibility is studied in the mixed lymphocyte reaction (MLR) induced by minor lymphocyte-stimulating (Mls) antigen-presenting irradiated B cells. By using anti-CD28 antibody, the CD28/B7-2-, LFA-1/ICAM-1-dependent Mls-MLR was found to be restored. By flow cytometry, approximately 70% B cells were lost but with unaffected B7-2 expression, indicating that the moderate CD28 costimulation was caused by mortality of antigen presenting cells. Despite of costimulatory deficiency, T cells were shown primed. However, the expression of early activation markers CD25 and CD69, which was shown unaffected by B7/CD28 blocking, was found partially inhibited. To further understand the regulation, we examined the ICAM-1 expression, and found that it was again not altered on irradiated B cells. Thus, the radiation-induced rapid loss of resting B cells may be the basic mechanism causing insufficient costimulatory activity in radiosensitive B-T interaction. Furthermore, the presence of an element, other than B7-2, involving in controlling early T-cell response is suggested. |
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| AbstractList | The regulation of CD28/B7 is important in T-cell activation. It has been argued that its aberrant expression is involved in the radiosensitivity of B-cell-stimulated T-cell response. Here, this possibility is studied in the mixed lymphocyte reaction (MLR) induced by minor lymphocyte-stimulating (Mls) antigen-presenting irradiated B cells. By using anti-CD28 antibody, the CD28/B7-2-, LFA-1/ICAM-1-dependent Mls-MLR was found to be restored. By flow cytometry, approximately 70% B cells were lost but with unaffected B7-2 expression, indicating that the moderate CD28 costimulation was caused by mortality of antigen presenting cells. Despite of costimulatory deficiency, T cells were shown primed. However, the expression of early activation markers CD25 and CD69, which was shown unaffected by B7/CD28 blocking, was found partially inhibited. To further understand the regulation, we examined the ICAM-1 expression, and found that it was again not altered on irradiated B cells. Thus, the radiation-induced rapid loss of resting B cells may be the basic mechanism causing insufficient costimulatory activity in radiosensitive B-T interaction. Furthermore, the presence of an element, other than B7-2, involving in controlling early T-cell response is suggested. |
| Author | Jiang, Shwu-Fen Chang, Kenneth S.S. Chow, Kai-Ping N. Shih, Charles C.-Y. Kong, Hong-Sheng Fu, Jin-Bau |
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| Copyright | 1998 S. Karger AG, Basel |
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| Issue | 5 |
| Keywords | Radiosensitivity B7 Costimulation T-cell activation |
| Language | English |
| License | Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. |
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| References | Van der Merwe PA, Bodian DL, Daenke S, Linsley P, Davis SJ. CD80 (B7-1) binds both CD28 and CTLA-4 with a low affinity and very fast kinetics. J Exp Med 185:393-403;1997. van Noesel C, Miedema F, Brouwer M, deRie MA, Aarden LA, van Lier RAW. Regulatory properties of LFA-1α and β chains in human T-lymphocyte activation. Nature 333:850-852;1988. Lucas PJ, Negishi I, Nakayama K, Fields LE, Loh DY. Naive CD28-deficient T cells can initiate but not sustain an in vitro antigen-specific immune response. J Immunol 154:5757-5768;1995. Prasad SA, Fling SP, Gregerson DS. A radiosensitive APC activity dissociates IL2 secretion and activation-induced cell death by autoreactive T cell hybridomas. Int Immunol 7:1787-1798;1995. Chambers CA, Allison JP. Co-stimulation in T cell responses. Curr Opi Immunol 9:396-404;1997. Noel PJ, Boise LH, Green JM, Thompson CB. CD28 costimulation prevents cell death during primary T cell activation. J Immunol 157:636-642;1996. Tivol EA, Borriello F, Schweitzer AN, Lynch WP, Bluestone JA, Sharpe AH. Loss of CTLA-4 leads to massive lymphoproliferation and fatal multiorgan tissue destruction, revealing a critical negative regulatory role of CTLA-4. Immunity 3:541-547;1995. Dougherty GJ, Murdoch S, Hogg N. The function of human intercelluar adhesion molecule-1 (ICAM-1) in the generation of an immune response. Eur J Immunol 18:35-39;1988. Hathcock KS, Laszlo G, Pucillo C, Linsley PS, Hodes RJ. Comparative analysis of B7-1 and B7-2 costimulatory ligands: Expression and function. J Exp Med 180:631-640;1994. Lollo BA, Chan KWH, Hanson EM, Moy VT, Brian AA. Direct evidence for two affinity state for lymphocyte function-associated antigen 1 on activated T cells. J Biol Chem 268:21693-21700;1993. Vyth-Dreese FA, Dellemijn TAM, Frijhoff A, van Kooyk Y, Figdor CG. Role of LFA-1/ICAM-1 in interleukin-2-stimulated lymphocyte proliferation. Eur J Immunol 23:3292-3299;1993. Ybarrondo B, O'Rourke AM, Brian AA, Mescher MF. Contribution of lymphocyte function-associated-1/intercelluar adhesion molecule-1 binding to the adhesion/signaling cascade of cytotoxic T lymphocyte activation. J Exp Med 179:359-363;1994. Mannie MD, Paterson PY, Thomas DW, Nairn R. Distinct accessory cell requirements define two types of rat T cell hybridomas specific for unique determinants in the encephalitogenic 68-86 region of myelin basic protein. J Immunol 144:440-450;1990. Walunas TL, Bakker CY, Bluestone JA. CTLA-4 ligation blocks CD28-dependent T cell activation. J Exp Med 183:2541-2550;1994. Krummel MF, Allison JP. CTLA-4 engagement inhibits IL2 accumulation and cell cycle progression upon activation of resting T cells. J Exp Med 183:2533-2540;1996. Van Seventer GA, Shimizu Y, Horgan KJ, Shaw S. The LFA-1 ligand ICAM-1 provides an important costimulatory signal for T cell receptor-mediated activation of resting T cells. J Immunol 144:4579-4586;1990. Hathcock KS, Laszlo G, Dickler HB, Bradshaw J, Linsley P, Hodes RJ. Identification of a novel CTLA-4 ligand that is costimulatory for T cell activation. Science 262:905-907;1993. Ashwell JD, Jenkins MK, Schwartz RH. Effect of gamma radiation on resting B lymphocytes. II. Functional characterization of the antigen-presentation defect. J Immunol 141:2536-2544;1988. Gajewski TF, Pinnas M, Wong T, Fitch FW. Murine Th1 and Th2 clones proliferate optimally in response to distinct antigen-presenting cell populations. J Immunol 146:1750-1758;1991. Kearney ER, Walunas TL, Karr RW, Morton PA, Loh DY, Bluestone JA, Jenkins MK. Antigen-dependent clonal expansion of a trace population of antigen specific CD4+ T cells in vivo is dependent on CD28 co-stimutaion and inhibited by CTLA-4. J Immunol 155:1032-1036;1995. Alegre M-L, Noel PJ, Eisfelder BJ, Chuang, E, Clark MR, Reiner SL, Thompson CB. Regulation of surface and intracellular expression of CTLA-4 on mouse T cells. J Immunol 157:4762-4770;1996. Goldbach-Mansky R, King PD, Taylor AP, Dupont B. A co-stimulatory role for CD28 in the activation of CD4+ T lymphoctes by staphylococcal enterotoxin B. Int Immunol 4:1351-1360;1992. Bachmann MF, McKall-Faienza K, Schmits R, Bouchard D, Beach J, Speiser DE, Mak TW, Ohashi PS. Distinct roles for LFA-1 and CD28 during activation of naive T cells: Adhesion versus costimulation. Immunity 7:549-557;1997. Von Boehmer H, Sprent J. Expression of M locus differences by B cells but not by T cells. Nature 249:363-365;1974. Azuma M, Ito D, Yagita H, Okumura K, Phillips JH, Lanier LL, Somoza C. B70 antigen is a second ligand for CTLA-4 and CD28. Nature 366:76-79;1993. Waterhouse P, Penninger JM, Timms E, Wakeham A, Shaninian A, Lee KP, Thompson CB, Griesser H, Mak TW. Lymphoproliferative disorders with early lethality in mice deficient in Ctla-4. Science 270:985-988;1995. Minami M, Shreffler DC, Cowing C. Characterization of the stimulator cells in the murine primary mixed leukocyte response. J Immunol 124:1314-1321;1980. Hamilos DL, Mascali JJ, Chesnut RW, Young RM, Ishioka G, Grey HM. The role of dendritic cells as stimulators of minor lymphocyte-stimulating locus-specific T cell responses in the mouse. I. Differential capacity of dendritic cells to stimulate minor lymphocyte-stimulating locus-reactive T cell hybridomas and the primary anti-minor lymphocyte-stimulating locus mixed lymphocyte reaction. J Immunol 142:1069-1078;1989. Comoli P, Montagna D, Moretta A, Zecca M, Locatelli F, Maccario R. Alloantigen-induced human lymphocytes rendered nonresponsive by a combination of anti-CD80 monoclonal antibodies and cyclosporin-A suppress mixed lymphocyte reaction in vitro. J Immunol 155:5506-5511;1995. Freeman GJ, Borriello F, Hodes RJ, Reiser H, Gribben JG, Ng JW, Kim J, Goldberg JM, Hathcock K, Laszlo G, Lombard LA, Wang S, Gray GS, Nadler LM, Sharpe AH. Murine B7-2, an alternative CTLA-4 counter-receptor that costimulates T cell proliferation and interleukin 2 production. J Exp Med 178:2185-2192;1993. Lenschow DJ, Walunas TL, Bluestone JA. CD28/B7 system of T cell costimulation. Ann Rev Immunol 14:233-258;1996. Linsley PS, Bradshaw J, Green J, Peach R, Bennett KL, Mittler RS. Intracellular trafficking of CTLA-4 and focal localization towards sites of TCR engagement. Immunity 4:535-543;1996. Walunas TL, Lenschow DJ, Bakker CY, Linsley PS, Freeman GJ, Green JM, Thompson CB, Bluestone JA. CTLA-4 can function as a negative regulator of T cell activation. Immunity 1:405-413;1994. Turcovski-Corrales SM, Fenton RG, Peltz G, Taub DD. CD28:B7 interactions promote T cell adhesion. Eur J Immunol 25:3087-3093;1995. Festenstein H. Pertinent features of M locus determinants including revised nomenclature and strain distribution. Transplantation 18:555-557;1973. Fling SP, Donoso LA, Gregerson DS. In vitro unresponsiveness to autologous sequences of the immunopathogenic autoantigen, S-antigen. J Immunol 147:483-489;1991. Hargreaves R, Logiou V, Lechler R. The primary alloresponse of human CD4+ T cells is dependent on B7 (CD80), augmented by CD58, but relatively uninfluenced by CD54 expression. Int Immunol 7:1505-1513;1995. Krummel MF, Allison JP. CD28 and CTLA-4 have opposing effects on the response of T cells to stimulation. J Exp Med 182:459-465;1995. Kuhlman P, Moy VT, Lollo BA, Brian AA. The accessory function of murine intercelluar adhesion molecule-1 in T lymphocyte activation. Contributions of adhesion and co-activation. J Immunol 146:1773-1782;1991. Webb SR, Li JH, Wilson DB, Sprent J. Capacity of small B cell-enriched populations to stimulate mixed lymphocyte reactions: Marked differences between irradiated vs. mitomycin C-treated stimulators. Eur J Immunol 15:92-96;1985. Harding FA, McArther JG, Gross JA, Raulet DH, Allison JP. CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones. Nature 356:607-609;1992. Gimmi CD, Freeman GJ, Gribben JG, Sugita K, Freedman AS, Morimoto C, Nadler LM. B-cell surface B7 provides a costimulatory signal that induces T cells to proliferate and secrete interleukin 2. Proc Natl Acad Sci USA 88:6575-6579; 1991. Larson-Sciard EL, Spetz-Hagberg AL, Casrouge A, Kourilsky P. Analysis of T cell receptor V beta gene usage in primary mixed lymphocyte reactions: Evidence for directive usage by different antigen-presenting cells and Mls-like determinants on T cell blasts. Eur J Immunol 20:1223-1229;1990. Dennig D, Lacerda J, Yan Y, Gasparetto C, O'Reilly RJ. ICAM-1 (CD54) expression on B lymphocytes is associated with their costimulatory function and can be increased by coactivation with IL1 and IL7. Cell Immunol 156:414-423;1994. Stack RM, Lenschow DJ, Gray GS, Bluestone JA, Fitch FW. IL-4 treatment of small splenic B cells induces costimulatory molecules B7-1 and B7-2. J Immunol 152:5723-5733;1994. Ashwell JD, DeFranco AL, Paul WE, Schwartz RH. Antigen presentation by resting B cells: Radiosensitivity of the antigen-presentation function and two distinct pathways of T cell activation. J Exp Med 159:881-905;1984. Nestle FO, Thompson C, Shimizu Y, Turka LA, Nickoloff BJ. Costimulation of superantigen-activated T lymphocytes by autologous dendritic cells is dependent on B7. Cell Immunol 156:220-229;1994. Mannie MD, Nairn R. Subset-specific co-stimulatory signals are required for IL-2 production but not growth inhibition responses by T cell hybrids specific for myelin basic protein. Cell Immunol 140:219-236;1992. Lenschow DJ, Su GH-T, Zuckerman LA, Nabavi N, Jellis CL, Gray GS, Miller J, Bluestone JA. Expression and functional significance of an additional ligand for CTLA-4. Proc Natl Acad Sci USA 90:11054-11058;1993. Springer TA, Dustin ML, Kishimoto TK, Marlin SD. The lymphocyte function-associated LFA-1, CD2, and LFA-3 molecules: Cell adhesion receptors of the immune system. Ann Rev Immunol 5:223-252;1987. Boise LH, Minn AJ, Noel PJ, June CH, Accavitti M, Lindsten T, Thompson CB. CD28 costimulation can promote T cell survival by enhancing the expression of Bcl-XL. Immunity 3:87-98;1995. Morokata T, Kato T, Igarashi O, Nariuchi H. Mechanism of enhanced antigen presentation by B cells activated with anti-µ |
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| Title | The Radiation-Sensitive Costimulatory Factors Involved in B-Cell-Dependent T-Cell Activation by Minor Lymphocyte Stimulating Antigen |
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