Treatment and Prevention of Rat Glioblastma by Immunogenic C6 Cells Expressing Antisense Insulin-Like Growth Factor I RNA

Rat C6 glioma cells express insulin-like growth factor I (IGF-I) and form rapidly growing tumors in syngeneic animals. When transfected with an episome-based vector encoding antisense IGF-I complementary DNA, these cells lost tumorigenicity. Subcutaneous injection of IGF-I antisense-transfected C6 c...

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Bibliographic Details
Published inScience (American Association for the Advancement of Science) Vol. 259; no. 5091; pp. 94 - 97
Main Authors Trojan, Jerzy, Johnson, Thomas R., Rudin, Susan D., Ilan, Judith, Tykocinski, Mark L., Ilan, Joseph
Format Journal Article
LanguageEnglish
Published American Society for the Advancement of Science 01.01.1993
Subjects
Antibodies
Cell lines
Cellular immunity
Glioma
Lymphocytes
Neuroblastoma
Neurons
Somatic cells
Tumor cell line
Tumors
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Summary:Rat C6 glioma cells express insulin-like growth factor I (IGF-I) and form rapidly growing tumors in syngeneic animals. When transfected with an episome-based vector encoding antisense IGF-I complementary DNA, these cells lost tumorigenicity. Subcutaneous injection of IGF-I antisense-transfected C6 cells into rats prevented formation of both subcutaneous tumors and brain tumors induced by nontransfected C6 cells. The antisense-transfected cells also caused regression of established brain glioblastomas when injected at a point distal to the tumor. These antitumor effects result from a glioma-specific immune response involving CD8$^+$ lymphocytes. Antisense blocking of IGF-I expression may reverse a phenotype that allows C6 glioma cells to evade the immune system.
ISSN:0036-8075
1095-9203