Is cytogenetic damage a biomarker for the risk of malignancy development in renal transplantation patients?

• Published in: Hipokrat Tıp Dergisi Vol. 2; no. 1; pp. 6 - 13
• Main Authors: Mutlu,Emel, Ünal,Aydın, Kiraz,Aslıhan, Taşdemir,Arzu, Kökenek Ünal,Tuba Dilay, Koçyiğit,İsmail, Sipahioğlu,Murat Hayri, Tokgöz,Bülent
• Format: Journal Article
• Language: English
• Published: Bandırma Onyedi Eylül Üniversitesi Tıp Fakültesi 01.01.2022
• Subjects: Sağlık Hizmetleri; Tıp; Türkiye; Balıkesir; Böbrek Nakli; Malignite; Micronucleus; Sitokinez Blok Mikronükleus Analizi; Renal Transplantation; Malignancy; Sitogenetik Hasar; Mikronükleus; Cytogenetic Damage; Cytokinesis-Block Micronucleus Assay
• DOI: 10.29228/HMJ.8
• ISSN: 2791-9935

Introduction The micronucleus (MN) formation in the peripheral blood lymphocytes can be usable as a biomarker for the risk of cancer development. In this study, we aimed to evaluate the relationship between the MN formation in peripheral lymphocytes and the development of malignancy in renal transplant patients. Materials and Methods Ten renal transplant patients with post-transplant malignancy were included in the study. The control group with renal transplantation consisted of 15 age and sex matched renal transplant patients without post-transplant malignancy. The healthy control group consisted of 12 individuals who had similar age and sex ratios as the other two groups. The cytokinesis-block micronucleus (CBMN) assay was used for MN analysis. Results The number of MN in mononuclear cells was significantly higher in renal transplant patients with or without malignancy than in healthy controls [7.5 (2.0-11.0), 5.0 (0-12.0), and 1.0 (0-9.0), respectively, p<0.001]. Similarly, the number of MN in binuclear cells was significantly higher in renal transplant patients with or without malignancy than in healthy controls [54.0 (8.0-199.0), 32.0 (0-182.0), and 10.0 (2.00- 29.00), respectively, p<0.001]. Although the difference was not statistically significant, the number of MN both in mononuclear cells and in binuclear cells was higher in renal transplant patients with malignancy than renal transplant patients without malignancy. Conclusion Increase in the number of MN in mononuclear and binuclear cells may be a promising biomarker for malignancy development after renal transplantation.