Methylation and Acetylation Characteristics of Cloned Bovine Embryos from Donor Cells Treated with 5-aza-2′-Deoxycytidine

• Published in: Biology of reproduction Vol. 72; no. 4; p. 944
• Main Authors: B.P. Enright, L.-Y. Sung, C.-C. Chang, X. Yang, X.C. Tian
• Format: Journal Article
• Language: English
• Published: Society for the Study of Reproduction 01.04.2005

Differentiated somatic cells and embryos cloned from somatic cells by nuclear transfer (NT) have higher levels of DNA methylation than gametes and early embryos produced in vivo. Reducing DNA methylation in donor cells before NT by treating them with chemicals such as the DNA methyl-transferase inhi...