Characterization of Active Site Structure in CYP121

• Published in: The Journal of biological chemistry Vol. 283; no. 48; p. 33406
• Main Authors: Kirsty J. McLean, Paul Carroll, D. Geraint Lewis, Adrian J. Dunford, Harriet E. Seward, Rajasekhar Neeli, Myles R. Cheesman, Laurent Marsollier, Philip Douglas, W. Ewen Smith, Ida Rosenkrands, Stewart T. Cole, David Leys, Tanya Parish, Andrew W. Munro
• Format: Journal Article
• Language: English
• Published: American Society for Biochemistry and Molecular Biology 28.11.2008

Mycobacterium tuberculosis (Mtb) cytochrome P450 gene CYP121 is shown to be essential for viability of the bacterium in vitro by gene knock-out with complementation. Production of CYP121 protein in Mtb cells is demonstrated. Minimum inhibitory concentration values for azole drugs against Mtb H37Rv w...