C-terminal Fragment of Presenilin Is the Molecular Target of a Dipeptidic γ-Secretase-specific Inhibitor DAPT (N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-Butyl Ester)
γ-Secretase is a multimeric membrane protein complex composed of presenilin (PS), nicastrin, Aph-1 and, Pen-2 that is responsible for the intramembrane proteolysis of various type I transmembrane proteins, including amyloid β-precursor protein and Notch. The direct labeling of PS polypeptides by t...
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Published in | The Journal of biological chemistry Vol. 281; no. 21; p. 14670 |
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Main Authors | , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
American Society for Biochemistry and Molecular Biology
26.05.2006
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Abstract | γ-Secretase is a multimeric membrane protein complex composed of presenilin (PS), nicastrin, Aph-1 and, Pen-2 that is responsible
for the intramembrane proteolysis of various type I transmembrane proteins, including amyloid β-precursor protein and Notch.
The direct labeling of PS polypeptides by transition-state analogue γ-secretase inhibitors suggested that PS represents the
catalytic center of γ-secretase. Here we show that one of the major γ-secretase inhibitors of dipeptidic type, N -[ N -(3,5-difluorophenacetyl)- l -alanyl]- S -phenylglycine t -butyl ester (DAPT), targets the C-terminal fragment of PS, especially the transmembrane domain 7 or more C-terminal region,
by designing and synthesizing DAP-BpB ( N -[ N -(3,5-difluorophenacetyl)- l -alanyl]-( S )-phenylglycine-4-(4-(8-biotinamido)octylamino)benzoyl)benzyl)methylamide), a photoactivable DAPT derivative. We also found
that DAP-BpB selectively binds to the high molecular weight γ-secretase complex in an activity-dependent manner. Photolabeling
of PS by DAP-BpB is completely blocked by DAPT or its structural relatives ( e.g. Compound E) as well as by arylsulfonamides. In contrast, transition-state analogue inhibitor L-685,458 or α-helical peptidic
inhibitor attenuated the photolabeling of PS1 only at higher concentrations. These data illustrate the DAPT binding site as
a novel functional domain within the PS C-terminal fragment that is distinct from the catalytic site or the substrate binding
site. |
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AbstractList | γ-Secretase is a multimeric membrane protein complex composed of presenilin (PS), nicastrin, Aph-1 and, Pen-2 that is responsible
for the intramembrane proteolysis of various type I transmembrane proteins, including amyloid β-precursor protein and Notch.
The direct labeling of PS polypeptides by transition-state analogue γ-secretase inhibitors suggested that PS represents the
catalytic center of γ-secretase. Here we show that one of the major γ-secretase inhibitors of dipeptidic type, N -[ N -(3,5-difluorophenacetyl)- l -alanyl]- S -phenylglycine t -butyl ester (DAPT), targets the C-terminal fragment of PS, especially the transmembrane domain 7 or more C-terminal region,
by designing and synthesizing DAP-BpB ( N -[ N -(3,5-difluorophenacetyl)- l -alanyl]-( S )-phenylglycine-4-(4-(8-biotinamido)octylamino)benzoyl)benzyl)methylamide), a photoactivable DAPT derivative. We also found
that DAP-BpB selectively binds to the high molecular weight γ-secretase complex in an activity-dependent manner. Photolabeling
of PS by DAP-BpB is completely blocked by DAPT or its structural relatives ( e.g. Compound E) as well as by arylsulfonamides. In contrast, transition-state analogue inhibitor L-685,458 or α-helical peptidic
inhibitor attenuated the photolabeling of PS1 only at higher concentrations. These data illustrate the DAPT binding site as
a novel functional domain within the PS C-terminal fragment that is distinct from the catalytic site or the substrate binding
site. |
Author | Takeshi Iwatsubo Naoto Watanabe Yuichi Morohashi Toshiyuki Kan Yumiko Okamura Tohru Fukuyama Yusuke Tominari Taisuke Tomita Haruhiko Fuwa Chihiro Sato Hideaki Natsugari |
Author_xml | – sequence: 1 fullname: Yuichi Morohashi – sequence: 2 fullname: Toshiyuki Kan – sequence: 3 fullname: Yusuke Tominari – sequence: 4 fullname: Haruhiko Fuwa – sequence: 5 fullname: Yumiko Okamura – sequence: 6 fullname: Naoto Watanabe – sequence: 7 fullname: Chihiro Sato – sequence: 8 fullname: Hideaki Natsugari – sequence: 9 fullname: Tohru Fukuyama – sequence: 10 fullname: Takeshi Iwatsubo – sequence: 11 fullname: Taisuke Tomita |
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Snippet | γ-Secretase is a multimeric membrane protein complex composed of presenilin (PS), nicastrin, Aph-1 and, Pen-2 that is responsible
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Title | C-terminal Fragment of Presenilin Is the Molecular Target of a Dipeptidic γ-Secretase-specific Inhibitor DAPT (N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-Butyl Ester) |
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