Proteomic comparison of outer membrane protein patterns of sessile and planktonic Pseudomonas aeruginosa cells

We have compared the outer membrane protein (OMP) pattern of sessile Pseudomonas aeruginosa cells with that of its stationary-phase free-floating counterparts. Two-dimensional electrophoretic gels were compared using computing scanning densitometry. The level of 103 OMPs (i.e. about 50% of the spots...

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Published inBiofilms Vol. 2; no. 1; pp. 27 - 36
Main Authors Seyer, Damien, Cosette, P., Siroy, A., Dé, E., Lenz, C., Vaudry, H., Coquet, L., Jouenne, T.
Format Journal Article
LanguageEnglish
Published Cambridge University Press (CUP) 01.01.2005
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Life Sciences
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Abstract We have compared the outer membrane protein (OMP) pattern of sessile Pseudomonas aeruginosa cells with that of its stationary-phase free-floating counterparts. Two-dimensional electrophoretic gels were compared using computing scanning densitometry. The level of 103 OMPs (i.e. about 50% of the spots that fulfilled our statistical selection threshold) was significantly and reproducibly different in biofilm organisms as compared with their planktonic counterparts. Some OMPs that specifically either accumulated or decreased were identified by mass spectrometry and the role of these proteins in the biofilm phenotype is discussed.
AbstractList We have compared the outer membrane protein (OMP) pattern of sessile Pseudomonas aeruginosa cells with that of its stationary-phase free-floating counterparts. Two-dimensional electrophoretic gels were compared using computing scanning densitometry. The level of 103 OMPs (i.e. about 50% of the spots that fulfilled our statistical selection threshold) was significantly and reproducibly different in biofilm organisms as compared with their planktonic counterparts. Some OMPs that specifically either accumulated or decreased were identified by mass spectrometry and the role of these proteins in the biofilm phenotype is discussed.
Author Seyer, Damien
Dé, E.
Vaudry, H.
Coquet, L.
Cosette, P.
Jouenne, T.
Lenz, C.
Siroy, A.
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  fullname: Jouenne, T.
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Snippet We have compared the outer membrane protein (OMP) pattern of sessile Pseudomonas aeruginosa cells with that of its stationary-phase free-floating counterparts....
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Title Proteomic comparison of outer membrane protein patterns of sessile and planktonic Pseudomonas aeruginosa cells
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