Harnessing biodiesel-producing microbes: from genetic engineering of lipase to metabolic engineering of fatty acid biosynthetic pathway
Microbial production routes, notably whole-cell lipase-mediated biotransformation and fatty-acids-derived biosynthesis, offer new opportunities for synthesizing biodiesel. They compare favorably to immobilized lipase and chemically catalyzed processes. Genetically modified whole-cell lipase-mediated...
Saved in:
Published in | Critical reviews in biotechnology Vol. 37; no. 1; pp. 26 - 36 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Taylor & Francis
01.02.2017
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Microbial production routes, notably whole-cell lipase-mediated biotransformation and fatty-acids-derived biosynthesis, offer new opportunities for synthesizing biodiesel. They compare favorably to immobilized lipase and chemically catalyzed processes. Genetically modified whole-cell lipase-mediated in vitro route, together with in vivo and ex vivo microbial biosynthesis routes, constitutes emerging and rapidly developing research areas for effective production of biodiesel. This review presents recent advances in customizing microorganisms for producing biodiesel, via genetic engineering of lipases and metabolic engineering (including system regulation) of fatty-acids-derived pathways. Microbial hosts used include Escherichia coli, Saccharomyces cerevisiae, Pichia pastoris and Aspergillus oryzae. These microbial cells can be genetically modified to produce lipases under different forms: intracellularly expressed, secreted or surface-displayed. They can be metabolically redesigned and systematically regulated to obtain balanced biodiesel-producing cells, as highlighted in this study. Such genetically or metabolically modified microbial cells can support not only in vitro biotransformation of various common oil feedstocks to biodiesel, but also de novo biosynthesis of biodiesel from glucose, glycerol or even cellulosic biomass. We believe that the genetically tractable oleaginous yeast Yarrowia lipolytica could be developed to an effective biodiesel-producing microbial cell factory. For this purpose, we propose several engineered pathways, based on lipase and wax ester synthase, in this promising oleaginous host. |
---|---|
AbstractList | Microbial production routes, notably whole-cell lipase-mediated biotransformation and fatty-acids-derived biosynthesis, offer new opportunities for synthesizing biodiesel. They compare favorably to immobilized lipase and chemically catalyzed processes. Genetically modified whole-cell lipase-mediated in vitro route, together with in vivo and ex vivo microbial biosynthesis routes, constitutes emerging and rapidly developing research areas for effective production of biodiesel. This review presents recent advances in customizing microorganisms for producing biodiesel, via genetic engineering of lipases and metabolic engineering (including system regulation) of fatty-acids-derived pathways. Microbial hosts used include Escherichia coli, Saccharomyces cerevisiae, Pichia pastoris and Aspergillus oryzae. These microbial cells can be genetically modified to produce lipases under different forms: intracellularly expressed, secreted or surface-displayed. They can be metabolically redesigned and systematically regulated to obtain balanced biodiesel-producing cells, as highlighted in this study. Such genetically or metabolically modified microbial cells can support not only in vitro biotransformation of various common oil feedstocks to biodiesel, but also de novo biosynthesis of biodiesel from glucose, glycerol or even cellulosic biomass. We believe that the genetically tractable oleaginous yeast Yarrowia lipolytica could be developed to an effective biodiesel-producing microbial cell factory. For this purpose, we propose several engineered pathways, based on lipase and wax ester synthase, in this promising oleaginous host. |
Author | Yan, Jinyong Yan, Yunjun Madzak, Catherine Han, Bingnan |
Author_xml | – sequence: 1 givenname: Jinyong surname: Yan fullname: Yan, Jinyong organization: Huazhong University of Science and Technology [Wuhan] – sequence: 2 givenname: Yunjun surname: Yan fullname: Yan, Yunjun organization: Huazhong University of Science and Technology [Wuhan] – sequence: 3 givenname: Catherine orcidid: 0000-0002-1413-0602 surname: Madzak fullname: Madzak, Catherine organization: Paris-Saclay Food and Bioproduct Engineering – sequence: 4 givenname: Bingnan surname: Han fullname: Han, Bingnan organization: Shanghai Jiao Tong University School of Medicine |
BackLink | https://hal.inrae.fr/hal-03313089$$DView record in HAL |
BookMark | eNqVjbFOxDAQRF0c4u6AT0ByS5GwG-O7HB1CoBSU9NEm2SRGiR3ZBpQv4LeRER0V1Uhvnmb2YmOdZSGuEXKFcLqFoypLrTEvAHWOCHda4UbsEs9SsRX7EN4AQONRn4ttcdDFQWm1E18VecshGDvIxrjOcOApW7zr3tvEZtN613C4l713sxzYcjStZDsYy-yT4no5mYUCy-jkzJEaN_1VeopxldSaLv2E1cbxZ2mhOH7SeinOepoCX_3mhbh5fnp9rLKRpnrxZia_1o5MXT281ImBUqigPH2g-o_7DYdXYTA |
ContentType | Journal Article |
Copyright | Distributed under a Creative Commons Attribution 4.0 International License |
Copyright_xml | – notice: Distributed under a Creative Commons Attribution 4.0 International License |
DBID | 1XC |
DOI | 10.3109/07388551.2015.1104531 |
DatabaseName | Hyper Article en Ligne (HAL) |
DatabaseTitleList | |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Engineering |
EndPage | 36 |
ExternalDocumentID | oai_HAL_hal_03313089v1 |
GroupedDBID | --- .GJ 00X 03L 0BK 0R~ 0VX 1XC 29F 30N 34G 36B 39C 3V. 4.4 53G 5GY 5VS 6J9 7X2 7X7 88A 88E 8AO 8FE 8FG 8FH 8FI 8FJ A8Z AAGME AAHBH AAIKC AAJMT AALDU AALIY AAMIU AAMNW AAOAP AAPUL AAPXX AAQRR AAYOK ABCCY ABDBF ABEFU ABFIM ABFMO ABJNI ABLIJ ABLKL ABPAQ ABTAH ABUWG ABXUL ABXYU ACBBU ACDHJ ACGEJ ACGFO ACGFS ACPRK ACQMU ACTIO ACZPZ ADBBV ADCVX ADGTB ADGTR ADOPC ADRBQ ADXPE AEGXH AEGYZ AEISY AENEX AEOZL AEPSL AEYOC AEYQI AFDYB AFKRA AFKVX AFRAH AFWLO AGDLA AHDZW AHMBA AIAGR AIJEM AJWEG AKBVH AKOOK ALIPV ALMA_UNASSIGNED_HOLDINGS ALQZU APEBS APNXG AQRUH ARAPS ATCPS AURDB AWYRJ BABNJ BBNVY BENPR BFWEY BGLVJ BHPHI BLEHA BPHCQ BVXVI CAG CCCUG CCPQU COF CS3 CWRZV DGEBU DKSSO DU5 E.- EAP EBC EBD EBS EDH EJD EMB EMK EMOBN EPL EST ESTFP ESX F5P FYUFA G8K H13 HCIFZ HGUVV HMCUK HZ~ I-F ITG ITH JEPSP KOO KRBQP KWAYT KYCEM LJTGL LK8 M0K M0L M1P M44 M4Z M7P ML0 MM. NUSFT O9- OWHGL P2P P62 PCLFJ PQQKQ PROAC PSQYO RNANH ROSJB RRB RTWRZ RWL S0X SV3 TAE TBQAZ TDBHL TFDNU TFL TFT TFW TN5 TQWBC TTHFI TUROJ TUS UHS UKHRP V1S Y6R ZGOLN ZY4 ~02 ~1N ~KM |
ID | FETCH-hal_primary_oai_HAL_hal_03313089v13 |
ISSN | 0738-8551 |
IngestDate | Fri Sep 06 12:43:34 EDT 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 1 |
Keywords | s:Biodiesel biosynthesis lipase metabolic engineering genetic engineering |
Language | English |
License | Distributed under a Creative Commons Attribution 4.0 International License: http://creativecommons.org/licenses/by/4.0 |
LinkModel | OpenURL |
MergedId | FETCHMERGED-hal_primary_oai_HAL_hal_03313089v13 |
ORCID | 0000-0002-1413-0602 0000-0002-1413-0602 |
PMID | 26526353 |
ParticipantIDs | hal_primary_oai_HAL_hal_03313089v1 |
PublicationCentury | 2000 |
PublicationDate | 2017-02 |
PublicationDateYYYYMMDD | 2017-02-01 |
PublicationDate_xml | – month: 02 year: 2017 text: 2017-02 |
PublicationDecade | 2010 |
PublicationTitle | Critical reviews in biotechnology |
PublicationYear | 2017 |
Publisher | Taylor & Francis |
Publisher_xml | – name: Taylor & Francis |
SSID | ssj0005175 |
Score | 4.3479056 |
Snippet | Microbial production routes, notably whole-cell lipase-mediated biotransformation and fatty-acids-derived biosynthesis, offer new opportunities for... |
SourceID | hal |
SourceType | Open Access Repository |
StartPage | 26 |
SubjectTerms | Life Sciences |
Title | Harnessing biodiesel-producing microbes: from genetic engineering of lipase to metabolic engineering of fatty acid biosynthetic pathway |
URI | https://hal.inrae.fr/hal-03313089 |
Volume | 37 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1ZS8NAEF5KfdEH8cSbRXwRidYkm8O34kE8Eax4vJTNRattUjRV9A_4Z_0RzmS3OWzB4yWEzWayyXzMzG5mviVkQ2Me90yTKzpngaKHpqHYNTVUdJUbluf5GEdgtsWF4VzrJ7fstlL5LGQt9RN323sfWVfyH61CG-gVq2T_oNlMKDTAOegXjqBhOP5Kxw5_QkuFs323HWM-YNBReimHK7Z1MdnOFUlvaRkJCMKaxa0gJyHEYLHT7oEvwyi0GyQAis5wl5AnEK1zr-3jk57fIogbURJuaPzKS7-Gs80TJNEprqjAPcnQGv6dWHs9aUdvsfSfeetdP3roZ8A95_47fyxVLOamU2w4DwONJNLlIgY4xlopIaQxtJ9IwQyaYJItJmlppc0WRDElbEoDbBRcuaBW-e4kNMGxinJRLKb3MayG0JnwRwXg9LopclSDIWePlvvMQZ6AU79qXh4cNc-OL07LVzPubqd-1mwBtGqaBvGCZb_AXH1MNW1mVslY3Tm4v8lTknZTYujsjUXFGY53Z-RokclaDg1Co9bgV0AaGjWmyKSc09C6AOg0qQTRDJkoMF3Oko8cqnQEVOkAqnsUgUolUGkBhTQOqQAqTWKaAfV7lxSoFIFKi0ClEqhzZPPosLHvKPiteoJppTn6-2nzpBrFUbBAQKjqu1bNNyyV61xlrm3thmAhAttSbeaxRbL-s7yl33RaJuM5cFdINXnqB6sQjybumlTjF1g9lKM |
link.rule.ids | 230,315,786,790,891,27957,27958 |
linkProvider | Library Specific Holdings |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Harnessing+biodiesel-producing+microbes%3A+from+genetic+engineering+of+lipase+to+metabolic+engineering+of+fatty+acid+biosynthetic+pathway&rft.jtitle=Critical+reviews+in+biotechnology&rft.au=Yan%2C+Jinyong&rft.au=Yan%2C+Yunjun&rft.au=Madzak%2C+Catherine&rft.au=Han%2C+Bingnan&rft.date=2017-02-01&rft.pub=Taylor+%26+Francis&rft.issn=0738-8551&rft.volume=37&rft.issue=1&rft.spage=26&rft.epage=36&rft_id=info:doi/10.3109%2F07388551.2015.1104531&rft_id=info%3Apmid%2F26526353&rft.externalDBID=HAS_PDF_LINK&rft.externalDocID=oai_HAL_hal_03313089v1 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0738-8551&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0738-8551&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0738-8551&client=summon |