Dynamics of Staphylococcus aureus Cas9 in DNA target Association and Dissociation

• Published in: EMBO reports Vol. 21
• Main Authors: Zhang, Siqi, Zhang, Qian, Hou, Xi‐miao, Guo, Lijuan, Wang, Fangzhu, Bi, Lulu, Zhang, Xia, Li, Hai‐hong, Wen, Fengcai, Xi, Xu‐guang, Huang, Xingxu, Shen, Bin, Sun, Bo
• Format: Journal Article
• Language: English
• Published: EMBO Press 13.08.2020
• Subjects: Life Sciences; CRISPR; Helicase; Transcription; DNA-protein interaction; Genomics; SaCas9; single molecule Subject Categories Chromatin

Staphylococcus aureus Cas9 (SaCas9) is an RNA-guided endonuclease that targets complementary DNA adjacent to a protospacer adjacent motif (PAM) for cleavage. Its small size facilitates in vivo delivery for genome editing in various organisms. Herein, using single-molecule and ensemble approaches, we...