Comparison of the interaction of dihydrocholesterol and cholesterol with sphingolipid or phospholipid Langmuir monolayers

• Published in: Colloids and surfaces, B, Biointerfaces Vol. 59; no. 22; pp. 81 - 86
• Main Authors: Desbat, Bernard, Lancelot, Eloïse, Krell, Tino, Nicolaï, Marie-Claire, Vogel, Fred, Chevalier, Michel, Ed, Fr, Ronzon, Eric
• Format: Journal Article
• Language: English
• Published: Elsevier 15.11.2011
• Subjects: Life Sciences; Pharmaceutical sciences; Santé publique et épidémiologie; Brewster angle microscopy; Monolayer; Cholesterol; Lipid; Dihydrocholesterol

The production protocol of many whole cell/virion vaccines involves an inactivation step with β-propiolactone (BPL). Despite the widespread use of BPL, its mechanism of action is poorly understood. Earlier work demonstrated that BPL alkylates nucleotide bases, but its interaction with proteins has n...