A CRISPR/Cas9-Based Study of ICgloRPCYG/I, a Gene That Regulates Pathogenicity, Conidial Yield, and Germination in IColletotrichum gloeosporioides/I
The filamentous fungus Colletotrichum gloeosporioides is the causative agent of one of the most serious diseases that damage plant fruit. In this study, we discovered and experimentally characterized a new gene in Colletotrichum gloeosporioides named CgloRPCYG. The CRISPR/Cas9 knockout mutant and co...
Saved in:
Published in | Agronomy (Basel) Vol. 13; no. 7 |
---|---|
Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
MDPI AG
01.06.2023
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | The filamentous fungus Colletotrichum gloeosporioides is the causative agent of one of the most serious diseases that damage plant fruit. In this study, we discovered and experimentally characterized a new gene in Colletotrichum gloeosporioides named CgloRPCYG. The CRISPR/Cas9 knockout mutant and complementary strain of CgloRPCYG were then obtained by polyethylene glycol (PEG)-mediated protoplast transformation to determine the related phenotypes. Compared with the wild-type strain and complementary mutant, the pathogenicity of the CRISPR/Cas9 knockout mutant was significantly decreased, the conidial yield was significantly reduced, and conidial germination was significantly delayed. These data indicate that CgloRPCYG contributes to pathogenicity, conidial yield, and germination in C. gloeosporioides. The successful application of the CRISPR/Cas9 system in C. gloeosporioides also confirms its utility in filamentous fungi for fundamental research and practical application. Furthermore, CgloRPCYG is a potential target gene for use in the development of plant protection technologies, such as spray-induced gene silencing, with the aim of controlling plant anthracnose disease caused by C. gloeosporioides. |
---|---|
AbstractList | The filamentous fungus Colletotrichum gloeosporioides is the causative agent of one of the most serious diseases that damage plant fruit. In this study, we discovered and experimentally characterized a new gene in Colletotrichum gloeosporioides named CgloRPCYG. The CRISPR/Cas9 knockout mutant and complementary strain of CgloRPCYG were then obtained by polyethylene glycol (PEG)-mediated protoplast transformation to determine the related phenotypes. Compared with the wild-type strain and complementary mutant, the pathogenicity of the CRISPR/Cas9 knockout mutant was significantly decreased, the conidial yield was significantly reduced, and conidial germination was significantly delayed. These data indicate that CgloRPCYG contributes to pathogenicity, conidial yield, and germination in C. gloeosporioides. The successful application of the CRISPR/Cas9 system in C. gloeosporioides also confirms its utility in filamentous fungi for fundamental research and practical application. Furthermore, CgloRPCYG is a potential target gene for use in the development of plant protection technologies, such as spray-induced gene silencing, with the aim of controlling plant anthracnose disease caused by C. gloeosporioides. |
Audience | Academic |
Author | Pu, Jin-Ji Xia, Yang Zhang, He Zhang, Meng-Ting Ye, Zi Sun, Rui-Qing Liu, Xiao-Mei Xia, Yu-Qi |
Author_xml | – sequence: 1 fullname: Zhang, He – sequence: 2 fullname: Xia, Yu-Qi – sequence: 3 fullname: Xia, Yang – sequence: 4 fullname: Zhang, Meng-Ting – sequence: 5 fullname: Ye, Zi – sequence: 6 fullname: Sun, Rui-Qing – sequence: 7 fullname: Liu, Xiao-Mei – sequence: 8 fullname: Pu, Jin-Ji |
BookMark | eNqVkLFOw0AMhk-oSBTozugHaJpLQ0kylghKtijt0gmdck5idDmj3GXoe_DA3MDA2t-Df1n6bPm_FwvLFoV4SuQmTQsZq35iy-MlSWWWvOTJjVhuZZZGz2mxW_zzd2Ll3JcMKpI0l9lS_OyhbKpj3cSlckX0qhxqOPpZX4A7qMrecFOX50NcrUHBAS3CaVAeGuxnozw6qJUfuEdLLfnLGkq2pEkZOBMaHSCrAzaNZJUntkA2bGVj0LOfqB3mEcINZPfNEzFpdHH1KG47ZRyu_vqD2Ly_ncqPqFcGP8l2AVVtKI0jtSGKjsJ8n-3yXMpteO1q4BdaUGfS |
ContentType | Journal Article |
Copyright | COPYRIGHT 2023 MDPI AG |
Copyright_xml | – notice: COPYRIGHT 2023 MDPI AG |
DOI | 10.3390/agronomy13071681 |
DatabaseTitleList | |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Agriculture |
EISSN | 2073-4395 |
ExternalDocumentID | A758800291 |
GeographicLocations | China |
GeographicLocations_xml | – name: China |
GroupedDBID | 2XV 5VS 7X2 7XC 8FE 8FH AADQD AAFWJ ABDBF ADBBV AFKRA AFPKN AFZYC ALMA_UNASSIGNED_HOLDINGS ATCPS BCNDV BENPR BHPHI CCPQU ECGQY GROUPED_DOAJ HCIFZ IAO ITC KQ8 M0K MODMG M~E OK1 PATMY PIMPY PROAC PYCSY |
ID | FETCH-gale_infotracacademiconefile_A7588002913 |
ISSN | 2073-4395 |
IngestDate | Fri Feb 02 04:43:49 EST 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 7 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-gale_infotracacademiconefile_A7588002913 |
ParticipantIDs | gale_infotracacademiconefile_A758800291 |
PublicationCentury | 2000 |
PublicationDate | 20230601 |
PublicationDateYYYYMMDD | 2023-06-01 |
PublicationDate_xml | – month: 06 year: 2023 text: 20230601 day: 01 |
PublicationDecade | 2020 |
PublicationTitle | Agronomy (Basel) |
PublicationYear | 2023 |
Publisher | MDPI AG |
Publisher_xml | – name: MDPI AG |
SSID | ssj0000913807 |
Score | 4.552598 |
Snippet | The filamentous fungus Colletotrichum gloeosporioides is the causative agent of one of the most serious diseases that damage plant fruit. In this study, we... |
SourceID | gale |
SourceType | Aggregation Database |
SubjectTerms | Comparative analysis Genetic engineering Germination Polyols |
Title | A CRISPR/Cas9-Based Study of ICgloRPCYG/I, a Gene That Regulates Pathogenicity, Conidial Yield, and Germination in IColletotrichum gloeosporioides/I |
Volume | 13 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV07T-NAEF5FpIECcTwEHIemQKIIJlEck7g0BhIjgUIIUlKhtb0QS5wtJU7Dr6C4H3wzu-sHVorcNVa08nu-7Dz8zbeMnfFeS4SIFSPohL5KUHq23zWsK276JOhm-VTveHi8Grx07ifWpFb7KrGWlql_GXyu7Cv5H6viGNqVumT_wbL5SXEAf6N9cYsWxu1aNnYa7sh7Ho5I5JYvbOMaXVIoqYGqRcR9_0hGQ3faxx08ydOUMtON8YwTGVmuQi8WJNM_S_AiUaCXCHDxjy4bSqbZAtZUXu8r3kzGjvRkzSFNSOJ_tvxN0iIioSQ5SqKQJp87rxz5Ou9z2T9BIS3d50epBpFXrQc5zCaKxDtdGk9RdYxrb1s-kti5xjjzw7qM0TYLupWiMt0MvYbTL2a_Ns49BkZL6pO3WDGWTd9mCabdVV7BNG2iUXL9nOi1MUlUC8VUtLYdzJ4ogiY5hHq7a1uYztevbx-Ho7xuRzqqqgs_vxv19Zuu0qxeQzv6Usgy3mHbOtcARwHnB6uJeJdtoSG03orYY38cUBBqFgACCSBI3qAAUNO7AA4EHiDwQA4e-AaeC8igAxI6eFAcQgk4EMVQAQ5UgNP09tnl3e3YHRj0UK_0jtM5D7ju5EhiQWJir8VbNA_YRozDhwx6QSAsLizTNlsdIWyOuYWwu2Gv1XmzWwE_YudrnvR47T1_ss0CaidsI50vxS-MIVP_VJv1VNZg_gJOUHc1 |
link.rule.ids | 315,786,790,870,27957,27958 |
linkProvider | ProQuest |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=A+CRISPR%2FCas9-Based+Study+of+ICgloRPCYG%2FI%2C+a+Gene+That+Regulates+Pathogenicity%2C+Conidial+Yield%2C+and+Germination+in+IColletotrichum+gloeosporioides%2FI&rft.jtitle=Agronomy+%28Basel%29&rft.au=Zhang%2C+He&rft.au=Xia%2C+Yu-Qi&rft.au=Xia%2C+Yang&rft.au=Zhang%2C+Meng-Ting&rft.date=2023-06-01&rft.pub=MDPI+AG&rft.issn=2073-4395&rft.eissn=2073-4395&rft.volume=13&rft.issue=7&rft_id=info:doi/10.3390%2Fagronomy13071681&rft.externalDocID=A758800291 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2073-4395&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2073-4395&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2073-4395&client=summon |