Purification of carbamoyl phosphate synthetase 1 liver and its regulation in response to ice-nucleation and subsequent whole-body freezing

• Published in: Molecular and cellular biochemistry Vol. 455; no. 1-2; p. 29
• Main Authors: Green, Stuart R, Storey, Kenneth B
• Format: Journal Article
• Language: English
• Published: Springer 01.05.2019
• Subjects: Ammonia; Carbonates; Chromatography; EDTA; Enzymes; Frogs; Glucose; Ligases; Liver; Lysine; Phosphates; Protein denaturation; Urea

Carbamoyl phosphate synthetase I (CPS1) represents an important regulatory enzyme of the urea cycle that mediates the ATP-driven reaction ligating ammonium, carbonate, and phosphate to form carbamoyl phosphate. The freeze-tolerant wood frog (Rana sylvatica or Lithobates sylvaticus) accumulates high...